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What are the advantages and disadvantages of using the two methods

What are the advantages and disadvantages of using the two methods

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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What are the advantages and disadvantages of using the two methods found in the attached picture. 

bllb. SDS-PAGE and Wetern bbt e lbad 15 uLof pre-steined SDs-PAGE marker into Lanel Gel 2. run at Z00 v for 3omin transfe to membrane by punning get at lo V for h -block membrane with milk load Z5 uL of Sample into lane 2,3,4 Gel irun at 200 v for 3omin i run at 200 U for 3omin. Erase Please place gel in container -mubate with I st antibady - add 20 mL Staining Solution Wash membiane. -miclowave 3o S, then incubate 15 min incubarte with 2nd antibady - Vecycle staininy olution. - add destain solution, Microwawe 30 S. Page 5 of NB * Transfer Uhit Manual Shows how to layer the Pads, Filter Paper, Menbrane + gel
Transcribed Image Text:bllb. SDS-PAGE and Wetern bbt e lbad 15 uLof pre-steined SDs-PAGE marker into Lanel Gel 2. run at Z00 v for 3omin transfe to membrane by punning get at lo V for h -block membrane with milk load Z5 uL of Sample into lane 2,3,4 Gel irun at 200 v for 3omin i run at 200 U for 3omin. Erase Please place gel in container -mubate with I st antibady - add 20 mL Staining Solution Wash membiane. -miclowave 3o S, then incubate 15 min incubarte with 2nd antibady - Vecycle staininy olution. - add destain solution, Microwawe 30 S. Page 5 of NB * Transfer Uhit Manual Shows how to layer the Pads, Filter Paper, Menbrane + gel
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