What are the advantages and disadvantages of using the two methods
Chapter14: Formula Method
Section: Chapter Questions
Problem 40SST
Related questions
Question
What are the advantages and disadvantages of using the two methods found in the attached picture.
![bllb. SDS-PAGE and Wetern bbt
e lbad 15 uLof pre-steined SDs-PAGE marker into Lanel Gel 2. run at Z00 v for 3omin
transfe to membrane by
punning get at lo V for h
-block membrane with milk
load Z5 uL of Sample into lane 2,3,4
Gel irun at 200 v for 3omin
i run at 200 U for 3omin.
Erase
Please
place gel in container
-mubate with I
st antibady
- add 20 mL Staining Solution
Wash membiane.
-miclowave 3o S, then incubate 15 min
incubarte with 2nd antibady
- Vecycle staininy olution.
- add destain solution, Microwawe 30 S.
Page 5 of NB
* Transfer Uhit
Manual Shows how to layer the
Pads, Filter Paper, Menbrane + gel](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fb5f5a489-6758-46b7-97af-73114a90d638%2Fd4686ae7-3dbd-4251-971d-82511eac7aa3%2Fy9gsh2k_processed.jpeg&w=3840&q=75)
Transcribed Image Text:bllb. SDS-PAGE and Wetern bbt
e lbad 15 uLof pre-steined SDs-PAGE marker into Lanel Gel 2. run at Z00 v for 3omin
transfe to membrane by
punning get at lo V for h
-block membrane with milk
load Z5 uL of Sample into lane 2,3,4
Gel irun at 200 v for 3omin
i run at 200 U for 3omin.
Erase
Please
place gel in container
-mubate with I
st antibady
- add 20 mL Staining Solution
Wash membiane.
-miclowave 3o S, then incubate 15 min
incubarte with 2nd antibady
- Vecycle staininy olution.
- add destain solution, Microwawe 30 S.
Page 5 of NB
* Transfer Uhit
Manual Shows how to layer the
Pads, Filter Paper, Menbrane + gel
Expert Solution
![](/static/compass_v2/shared-icons/check-mark.png)
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Recommended textbooks for you
![Biology: The Unity and Diversity of Life (MindTap…](https://www.bartleby.com/isbn_cover_images/9781337408332/9781337408332_smallCoverImage.gif)
Biology: The Unity and Diversity of Life (MindTap…
Biology
ISBN:
9781337408332
Author:
Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:
Cengage Learning
Essentials of Pharmacology for Health Professions
Nursing
ISBN:
9781305441620
Author:
WOODROW
Publisher:
Cengage
![Biology: The Unity and Diversity of Life (MindTap…](https://www.bartleby.com/isbn_cover_images/9781337408332/9781337408332_smallCoverImage.gif)
Biology: The Unity and Diversity of Life (MindTap…
Biology
ISBN:
9781337408332
Author:
Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:
Cengage Learning
Essentials of Pharmacology for Health Professions
Nursing
ISBN:
9781305441620
Author:
WOODROW
Publisher:
Cengage