What are the advantages and disadvantages of using the two methods

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question

What are the advantages and disadvantages of using the two methods found in the attached picture. 

bllb. SDS-PAGE and Wetern bbt
e lbad 15 uLof pre-steined SDs-PAGE marker into Lanel Gel 2. run at Z00 v for 3omin
transfe to membrane by
punning get at lo V for h
-block membrane with milk
load Z5 uL of Sample into lane 2,3,4
Gel irun at 200 v for 3omin
i run at 200 U for 3omin.
Erase
Please
place gel in container
-mubate with I
st antibady
- add 20 mL Staining Solution
Wash membiane.
-miclowave 3o S, then incubate 15 min
incubarte with 2nd antibady
- Vecycle staininy olution.
- add destain solution, Microwawe 30 S.
Page 5 of NB
* Transfer Uhit
Manual Shows how to layer the
Pads, Filter Paper, Menbrane + gel
Transcribed Image Text:bllb. SDS-PAGE and Wetern bbt e lbad 15 uLof pre-steined SDs-PAGE marker into Lanel Gel 2. run at Z00 v for 3omin transfe to membrane by punning get at lo V for h -block membrane with milk load Z5 uL of Sample into lane 2,3,4 Gel irun at 200 v for 3omin i run at 200 U for 3omin. Erase Please place gel in container -mubate with I st antibady - add 20 mL Staining Solution Wash membiane. -miclowave 3o S, then incubate 15 min incubarte with 2nd antibady - Vecycle staininy olution. - add destain solution, Microwawe 30 S. Page 5 of NB * Transfer Uhit Manual Shows how to layer the Pads, Filter Paper, Menbrane + gel
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

Blurred answer
Knowledge Booster
Forensic Analysis
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON