The BSA stock solution from the previous problem was then diluted to generate a set of standardsolutions of known concentrations. After performing biuret assay on these solutions, their absorbanceat 540 nm were measured using a UV-vis spectrophotometer. The following data were obtained.Concetration of BSAAbsorbance(mg/mL)0.10.0480.20.0950.40.1910.60.2900.80.3801.00.485Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of anunknown sample having an absorbance of 0.325.

CONCENTRATION OF BSA (mg/ml) ABSORBANCE 0 0 0.1 0.048 0.2 0.095 0.4 0.191 0.6 0.290 0.8…

Answered: The BSA stock solution from the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Apple puree was analyzed for petulin by HPLC-MS-MS after SPE clean-up. The procedure was 10.0g of puree + 10μl of a 10μg/ml solution of isotopically labeled petulin as internal standard were treated with 10.0ml of pectinase and acetic acid, centrifuged, and filtered. Four ml of the filtrate was passed through a SPE cartridge. The petulin was eluted with 2.0ml of ethyl acetate. The sample was evaporated to dryness and the residue dissolved in 1.0ml of the mobile phase. The analyte signal was 127 and the internal standard 197. Calculate the concentration of petulin in the sample in μg/g (RRF=1).
the figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.
A solution of flycine and a solution of albumin were prepared with the same concentration (g/L). One of the soutions turned light purple and the other dark purple for hte ninhydrin test. Which one would you expect to give the darker purple color? Explain.
Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the total magnification of the image under microscope? Show your solution.
Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that a bacterial culture contains 7.4 x 109 CFUs/ml. What dilution of this sample would give you a countable plate?
Assume that you have an “X” solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. Prepare a standard curve by using the absorbance of known concentrations (Hint: you can learn how to prepare a standard curve by using Excell from youtube). Calculate the concentration of unknown.
Explain the color changes observed throughout the peroxide value of oil experiment. Materials:1. Old and fresh oil samples2. Distilled water3. Acetic acid/chloroform solution4. Saturated potassium iodide solution5. Sodium thiosulphate (0.01M)6. 1% Starch indicator: dissolve 1g of starch in 10 ml of water and then add 90 ml of hotwater to it
What is the estimated size of the specimen under a 10x10 ocular micrometer under HPO? (Take note: specimen Occupies 10 OU) Where: 1 OU = 25µ
If you transferred 15 mL of an undiluted sample into 35 mL of diluting fluid, what dilution have you created? (Note: provide your answer in decimal format to three decimal places.)
If I am making 500ml of a solution with 0.625ml ITS, 0.0035ml Linoleic acid, 20ml Nu-serum, 0.75g BSA, and DMEM:F12 as my solvent, how best would I make this solution and what tools would be the most accurate to use?
Make a standard curve by using the values given in the table and determine the concentration of unknown sample? Tube 0,5 µg/ µl BSA 0,15 M NaCl Comassi Brillant Blue Solution Absorbance 595 nm 1 0 100 µl 900 µl 0 2 10 µl 90 µl 900 µl 0,105 3 20 µl 80 µl 900 µl 0,233 4 30 µl 70 µl 900 µl 0,41 5 40 µl 60 µl 900 µl 0,455 6 50 µl 50 µl 900 µl 0,589 Unknown Sample 10 µl 90 µl 900 µl 1,2
Normal text Calibri BI UA G |EE| +2 I +3+1 CICCO4, 1 LE | ZJ VITICHI SUpstances did not test positive for any of the organic compound Distilled water did not test positive for any of the organic compounds. 5| 6 3) 4) What do all of the indicators you have used have in common? Indicators are all substances whose solutions change color due to changes in p 5) Is there a macromolecule that the tests in this lab did not test for No, there is no macromolecule that the lab did not test for. 6) Which compound is most common in foods that come from plants? Cellocuse is the single most common biochemical compound in living things. 7) If you wanted to reduce the amount of fat in your diet, what foods would you avoid? Steer away from foods high in saturated and trans fats like fried foods, fatty meats, stick margarine, snacks and items with hydrogenated oil. M 6 8) Which foods tested would your body use for a quick burst of energy? a. For energy when no carbohydrates are available? b. For building…

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