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BglII 6. You have a circular plasmid available, with a single RE site for BglII (A^GATCT). You cleave the plasmid with BglII, and join it to both ends of a linear fragment of viral DNA which was obtained by digesting the viral genome with the RE DpnII (^GATC), resulting in a larger plasmid. ☑ DpnII Text DpnII O If the resulting larger plasmid is incubated with BglII, will the enzyme be able to excise the fragment of viral DNA? If no, why not? If yes, explain under which conditions. Motivate your answer by showing the sequence of the plasmid DNA (in black) and the viral DNA (in blue) at both the join positions. Write out the sequences in dsDNA format, if necessary indicate any flanking nucleotides by N.

BglII 6. You have a circular plasmid available, with a single RE site for BglII (A^GATCT). You cleave the plasmid with BglII, and join it to both ends of a linear fragment of viral DNA which was obtained by digesting the viral genome with the RE DpnII (^GATC), resulting in a larger plasmid. ☑ DpnII Text DpnII O If the resulting larger plasmid is incubated with BglII, will the enzyme be able to excise the fragment of viral DNA? If no, why not? If yes, explain under which conditions. Motivate your answer by showing the sequence of the plasmid DNA (in black) and the viral DNA (in blue) at both the join positions. Write out the sequences in dsDNA format, if necessary indicate any flanking nucleotides by N.

Biochemistry
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter28: Dna Metabolism: Replication, Recombination, And Repair
Section: Chapter Questions
Problem 4P: Multiple Replication Forks in E. coli II On the basis of Figure 28.2, draw a simple diagram...
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BglII 6. You have a circular plasmid available, with a single RE site for BglII (A^GATCT). You cleave the plasmid with BglII, and join it to both ends of a linear fragment of viral DNA which was obtained by digesting the viral genome with the RE DpnII (^GATC), resulting in a larger plasmid. ☑ DpnII Text DpnII O If the resulting larger plasmid is incubated with BglII, will the enzyme be able to excise the fragment of viral DNA? If no, why not? If yes, explain under which conditions. Motivate your answer by showing the sequence of the plasmid DNA (in black) and the viral DNA (in blue) at both the join positions. Write out the sequences in dsDNA format, if necessary indicate any flanking nucleotides by N.
Transcribed Image Text:BglII 6. You have a circular plasmid available, with a single RE site for BglII (A^GATCT). You cleave the plasmid with BglII, and join it to both ends of a linear fragment of viral DNA which was obtained by digesting the viral genome with the RE DpnII (^GATC), resulting in a larger plasmid. ☑ DpnII Text DpnII O If the resulting larger plasmid is incubated with BglII, will the enzyme be able to excise the fragment of viral DNA? If no, why not? If yes, explain under which conditions. Motivate your answer by showing the sequence of the plasmid DNA (in black) and the viral DNA (in blue) at both the join positions. Write out the sequences in dsDNA format, if necessary indicate any flanking nucleotides by N.
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