a) What is the difference between blunt ends and sticky ends and which one is useful for vector cloning? b) There are also possible outcomes in terms of the attempt to get the bacteria to pick up the recombinant plasmid: Some bacteria will not pick up the plasmid Some bacteria will pick up the recombinant plasmid. Describe how scientists target ONLY the bacteria that have the recombinant plasmid. c) IPTE xgal is a lactose analog that Is used in lactose free milk Is used to prevent growth of bacteria that do not have the beta galactosidase gene Is used to differentiate bacteria that have picked up a target DNA within their plasmids from those that have not Helps with bacterial transformation
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
a) What is the difference between blunt ends and sticky ends and which one is useful for vector cloning?
b) There are also possible outcomes in terms of the attempt to get the bacteria to pick up the recombinant plasmid:
Some bacteria will not pick up the plasmid
Some bacteria will pick up the recombinant plasmid.
Describe how scientists target ONLY the bacteria that have the recombinant plasmid.
c) IPTE xgal is a lactose analog that
-
- Is used in lactose free milk
- Is used to prevent growth of bacteria that do not have the beta galactosidase gene
- Is used to differentiate bacteria that have picked up a target DNA within their plasmids from those that have not
- Helps with bacterial transformation
Step by step
Solved in 2 steps
