A scientist uses transduction to map genes in a strain of E. coli. For this experiment, the donor strain is leu+ phe+lys-+arg+. The recipient is leu-phe-lys-arg- Co-transduction rates were • leut and phe+ = 29% • leu+ and arg+ = 46% • leu+ and lys+ = 6% Which gene is furthest from leu? lys phe arg leu Is the percentage is the chance of being co-transduction

A scientist uses transduction to map genes in a strain of E. coli. For this experiment, the donor strain is leu+ phe+lys-+arg+. The recipient is leu-phe-lys-arg- Co-transduction rates were • leut and phe+ = 29% • leu+ and arg+ = 46% • leu+ and lys+ = 6% Which gene is furthest from leu? lys phe arg leu Is the percentage is the chance of being co-transduction

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Description: A scientist uses transduction to map genes in a strain of E. coli. For this experiment, the donor strain is leu+ phe+lys-+arg+. The recipient is leu-phe-lys-arg- Co-transduction rates were • leut and phe+ = 29% • leu+ and arg+ = 46% • leu+ and lys+ =

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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A general transduction experiment was conducted to determine the gene order of trp, pyrF and qts loci on the bacterial chromosome. The Donor genotype is: trp+pyr+qts The Recipient genotype is: trp pyr qts+ The selected marker in this transduction experiment is trp+. Unselected Markers Number of colonies pyr+qts+ 22 pyr+qts 10 pyr qts+ 68 pyr qts 0 What is the full genotype of each of the 22 colonies for the 3 loci? What is the gene order of the 3 loci on the donor chromosome? How is co-transduction relevant to determining the answer to part b? Why is the name of the viral vector not given to help you answer this question?
Determine the map distances between the genes.
Jamie conducts an experiment to determine the order of gene in bacteria. She uses an Hfr bacteria which is his", leu", mal * and gal*. If the recipient is auxotroph for all these genes and she selectively choose gal * and get the following pattern: 100% are gal* 60% are his 0% are mal* 20% are leu * What would be the gene order and indicate where in the genome is the origin of transfer?
The following recombinants are recovered when conjugation occurs between an a+d+g+ donor and an a d'g recipient. at dt g+= 84% ad g+= 6% at dg+ = 10% ad g+= less than 1% What is the map distance between the a and d genes? 6 map units less than 1 map unit 10 map units 84 map units 74 map units
In E. coli, the gene bioD+ encodes an enzyme involved in biotin synthesis, and galK+ encodes an enzyme involved in galactose utilization. An E. coli strain that contained wild-type versions of both genes was infected with P1 phage, and then a P1 lysate was obtained. This lysate was used totransduce (infect) a strain that was bioD− and galK−. The cellswere plated on a medium containing galactose as the sole carbonsource for growth to select for transduction of the galK+ gene.This medium also was supplemented with biotin. The resultingcolonies were then restreaked on a medium that lacked biotin tosee if the bioD+ gene had been cotransduced. The following resultswere obtained:What information do you know based onthe question and your understanding of the topic?
In E. coli, the gene bioD+ encodes an enzyme involved in biotin synthesis, and galK+ encodes an enzyme involved in galactose utilization. An E. coli strain that contained wild-type versions of both genes was infected with P1 phage, and then a P1 lysate was obtained. This lysate was used totransduce (infect) a strain that was bioD− and galK−. The cellswere plated on a medium containing galactose as the sole carbonsource for growth to select for transduction of the galK+ gene.This medium also was supplemented with biotin. The resultingcolonies were then restreaked on a medium that lacked biotin tosee if the bioD+ gene had been cotransduced. The following resultswere obtained:What topic in genetics does this question address?
Bacteriophage P22 was used in generalised transduction experiments to infect the Salmonella typhimurium donor strains described in the table below. The resulting phage lysates were then used to infect the recipient strains of S. typhimurium recipient strains listed in the table. In each cross, a phenotype was selected for one of the selected for one of the three genetic markers studied (str, aceA, thrA), and were made to select the recombinants corresponding to the other two markers. markers. The results are given in the following table: Strain I donor str thrA aceA thrA str aceA+ Strain recipient strs thrA+ aceA thrA str aceA Phenotype selected Str Ace+ Str recombinants selected ThrA ThrA ThrA ThrA Ace Ace Number 60 40 95 5 10 90 str: gene involved in streptomycin resistance, aceA: gene involved in the use of acetate as a carbon source, thrA: gene involved in threonine biosynthesis. 1) What are the selective media used in these three transduction experiments? to obtain the selected…
A transduction experiment was carried out to map the his and leu genes, which are involved in amino acid biosynthesis, of E. coli using a his+ leu+ donor and a his- leu- recipient. his+ leu- transductants will grow on agar media with: OA lacking histidine, containing leucine O B. containing histidine, containing leucine OC containing histidine, lacking leucine O D.a and b are correct O E. a, b and c are correct
Wild-type bacteria were used as donors to transduce the following recipient genotypes with the results indicated as the number of wild type colonies: Recipient Recipient leu- phe+ his- met+ tyr+ leu- phe+ his+ met- tyr+ leu- phe+ his+ met+ tyr- leu- phe- his+ met+ tyr+ leu+ phe+ his-met- tyr+ 127 leu+ phe-his- met+ tyr+ #wild type 37 138 132 1 leu+ phe+ his- met+ tyr- leu+ phe-his+ met- tyr+ leu+ phe-hist met+ tyr- leu- phe+ his+ met+ tyr+ 22 6 leu+ phe+ his+ met- tyr- 148 31 304 leu+ phe+ his+ met+ tyr- What is the order of the genes? tyr-leu-his-phe-met O leu-met-phe-his-tyr leu-his-met-tyr-phe his-tyr-leu-phe-met #wild type 356 10
In a generalized-transduction experiment, phages arecollected from an E. coli donor strain of genotype cys+leu+ thr+ and used to transduce a recipient of genotypecys- leu- thr-. Initially, the treated recipient populationis plated on a minimal medium supplemented with leucine and threonine. Many colonies are obtained.a. What are the possible genotypes of these colonies?b. These colonies are then replica plated onto threedifferent media: (1) minimal plus threonine only, (2)minimal plus leucine only, and (3) minimal. Whatgenotypes could, in theory, grow on these three media?c. Of the original colonies, 56 percent are observed togrow on medium 1, 5 percent on medium 2, and nocolonies on medium 3. What are the actual genotypes ofthe colonies on media 1, 2, and 3?d. Draw a map showing the order of the three genes andwhich of the two outer genes is closer to the middle gene
In a transformation experiment involving a wild type bacterial strain with a recipient strain with mutations in genes f, g, h and i pairs of genes were analyzed for co-transformation with the following results: Gene Pair Co-transformation g+ i+ f+ i+ yes no f+ h+ Уes f+ g+ no g+ h+ h+ i+ no уes What is the linear order of these genes relative to cach other?
In Figure 5-14, which is the last gene to be transferredinto the F– from each of the five Hfr strains?