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- 1. If you create your own species using recombinant genetics, what organism would you can create?2. The telomeric sequence in a species is (5-AACGGT-3'), repeats. You design a FISH experiment with the probe 5-AACGGTAACGGTAACGGTAACGGT-3. Which of the following illustrates the expected image of a metacentric chromosome (the dark region indicates fluorescence)? a. C. XX X e. d.1. How does site specific recombination differ from homologous recombination? a. It requires a small and defined sequence similarity between two DNA segments that will recombine. b. It requires a large sequence similarity between two DNA segments that will recombine. c. It involves exonucleases that will recognize the two DNA segments that will recombine. d. It involves endonucleases that will recognize the two DNA segments that will recombine. 2. What will be the consequence if the exonuclease domain of DNA polymerase III is mutated and rendered nonfunctional? a. The polymerase can still elongate the DNA template. b. Mutations due to replication errors will increase. c. There will be no synthesis of the lagging strand d. The primers in the lagging strand will not be removed. 3. Which of the following statements best explains the mechanism for DNA replication? a. It is reductive because half the total DNA present is copied. b. It is conservative because one resulting molecule…
- 6. A research group has selected three independent Trp−haploid strains of Neurospora, each of which cannotgrow in the absence of the amino acid tryptophan.They first mated these three strains with a wild-typestrain of opposite mating type, and then they analyzedthe resultant octads. For all three matings, two of thefour spore pairs in every octad could grow on minimalmedium (that is, in the absence of tryptophan), whilethe other two spore pairs were unable to grow on thisminimal medium.a. What can you conclude from this result?In the matings of mutant strains 1 and 2 with wild type,one of the two topmost pairs in some octads had sporesthat could grow on minimal medium while the other ofthe two topmost pairs in the same octads had spores thatcould not grow on minimal medium. In the mating ofmutant strain 3 with wild type, either all the spores inthe two topmost pairs could grow on minimal mediumor all could not grow on minimal medium.b. What can you conclude from this result?The…What do you mean by In Situ Hybridization?Help
- Please asapDescribe about fluorescence in situ hybridization (FISH).4. Analysis of four double-stranded DNA samples yielded the following information: Sample 1-35% cytosine Sample 3- 15% thymine Sample 4- 12% adenine Sample 2- 28% guanine 4.1 What would be the percentages of the other bases in each sample? 4.2 Could any of these samples come from the same organism?
- 4. The following distance matrix was provided for five nucleotide sequences. A B D A 8 7 12 15 10 9 18 9. D 13 E From the matrix, construct a scaled Phylogenetic tree using the UPGMA method. Show calculations for each step. B.4. What is the name of the process by which bacteria pick up a different organism’s geneticmaterial?5. Genetically, how does the original bacterial DNA (plasmid) differ from the final bacterial DNAmolecule? (Do not say, “It is longer.”)6. Tetracycline is an antibiotic that is prescribed to kill bacterial infections. The transformedbacterial cell that you created has the tetracycline resistant gene in it. If this cell is placed on agrowth medium with tetracycline, will the bacteria grow or die? Explain.Explain why a solid state fermentation system is optimal for extracellular enzyme production from fungi? 7. You have discovered a secondary metabolite made by an unusual fungus that has great industrial potential. Unfortunately, the fungus makes very of this product, so you must velop a scheme to increase the yield. Can you think of using a technique that might be useful to enhance the productivity of that secondary metabolite?
