A Results: Gene targeting of ES cells chromosome 1 lefty2 wild type allele targeting vector 16 kb lefty2 leftyl HH HI 5'probe X H H HH L Flanking DNA homology -ve Selectable marker HHH neo DT] +ve Selectable marker 12 kb H targeted allele B diagnostic restriction fragment length H for recombinant H H H neo Result: heterozygous and homozygous mutants 1kb 16 kb 12 kb

A Results: Gene targeting of ES cells chromosome 1 lefty2 wild type allele targeting vector 16 kb lefty2 leftyl HH HI 5'probe X H H HH L Flanking DNA homology -ve Selectable marker HHH neo DT] +ve Selectable marker 12 kb H targeted allele B diagnostic restriction fragment length H for recombinant H H H neo Result: heterozygous and homozygous mutants 1kb 16 kb 12 kb

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...

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Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant sickle cell anemia allele of beta globin. This mutation destroys an MstII restriction site normally present in the beta globin gene. This difference between the normal allele and the mutant allele can be detected with Southern blotting. Using a labeled beta globin gene as a probe, what differences would you expect to see for a Southern blot of the normal beta globin gene and the mutant sickle cell gene?
Heteroduplex DNA Formation in Recombination From the information in Figures 28.17 and 28.18, diagram the recombinational event leading to the formation of a heteroduplex DNA region within a bacteriophage chromosome.
Homologous Recombination, Heteroduplex DNA, and Mismatch Repair Homologous recombination in E. coli leads to the formation of regions of heteroduplex DNA. By definition, such regions contain mismatched bases. Why doesnâ€™t the mismatch repair system of E. coli eliminate these mismatches?
What Art the Features of the Series of -omes? Define the following terms: a. Genome b. Transcriptome c. Proteome d. Metabolome e. Fluxome
Transcribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’
Transcribe and translate the following DNA sequence (nontemplate strand): 5'- ATGGCCGGTTATTAAGCA-3'
Match each term with the most suitable description. ______ DNA profile a. GMO with a foreign gene ______ Ti plasmid b. alleles commonly have them ______ probes c. unique array of short tandem repeats ______ SNPs d. used to find clones. ______ transgenic e. mouse vs. human ______ genomics f. used in plant gene transfers ______ CRISPR g. based on RNA interference
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Which of the following sequences on a DNA molecule would be complementary to GCTTATAT? TAGGCGCG ATCCGCGC CGAATATA TGCCTCTC
Which of the following does the enzyme primase synthesize? DNA primer RNA primer Okazaki fragments phosphodiester linkage
Multiple Replication Forks in E. coli I Assuming DNA replication proceeds at a rate of 750 base pairs per second, calculate how long it will take to replicate the entire E. coli genome. Under optimal conditions, E. coli cells divide every 20 minutes. What is the minimal number of replication forks per E. coli chromosome in order to sustain such a rate of cell division?
Match each term with the most suitable description. ____ DNA profile a. GMO with a foreign gene ____ Ti plasmid b. alleles commonly have them ____ eugenics c. a persons unique collection of short tandem repeats ____ SNPs d. selecting desirable traits ____ transgenic e. genetically modified ____ GMO f. used in plant gene transfers