A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: [Substrate] (mm) 10 30 50 70 ▪ slope = [Select] ▪ Vmax= ▪ Km= Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor. ▪ y-int= [Select] [Select] . [Select] ▪ Km= slope = ▪ Vmax= [Select] [Select] Vo (mm/min) (without inhibitor) 4.43 10.26 14.07 16.73 [Select] Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of an inhibitor. ▪ y-int= [Select] What is the type of inhibition involved? mM/min mM Vo (mm/min) (with inhibitor) ✓mM/min mM 2.60 6.06 [Select] 8.23 9.73
A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: [Substrate] (mm) 10 30 50 70 ▪ slope = [Select] ▪ Vmax= ▪ Km= Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor. ▪ y-int= [Select] [Select] . [Select] ▪ Km= slope = ▪ Vmax= [Select] [Select] Vo (mm/min) (without inhibitor) 4.43 10.26 14.07 16.73 [Select] Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of an inhibitor. ▪ y-int= [Select] What is the type of inhibition involved? mM/min mM Vo (mm/min) (with inhibitor) ✓mM/min mM 2.60 6.06 [Select] 8.23 9.73
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below:
[Substrate]
(MM)
10
30
50
70
▪ slope =
▪ Vmax=
▪ Km =
Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor.
▪y-int= [Select]
[Select]
▪ Km =
[Select]
[Select]
Vo (mm/min)
(without inhibitor)
[Select]
4.43
10.26
14.07
16.73
[Select]
mM/min
mM
Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of an inhibitor.
▪ y-int= [Select]
▪ slope = [Select]
▪ Vmax=
Vo (mm/min)
(with inhibitor)
✓mM/min
mM
2.60
6.06
8.23
9.73
What is the type of inhibition involved? [Select]](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F60de6dac-069d-43b3-b08f-175b617e03fc%2F37e6e73e-39f6-4e51-ad08-d0797d3e27e0%2F7lwcof_processed.png&w=3840&q=75)
Transcribed Image Text:A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below:
[Substrate]
(MM)
10
30
50
70
▪ slope =
▪ Vmax=
▪ Km =
Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor.
▪y-int= [Select]
[Select]
▪ Km =
[Select]
[Select]
Vo (mm/min)
(without inhibitor)
[Select]
4.43
10.26
14.07
16.73
[Select]
mM/min
mM
Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of an inhibitor.
▪ y-int= [Select]
▪ slope = [Select]
▪ Vmax=
Vo (mm/min)
(with inhibitor)
✓mM/min
mM
2.60
6.06
8.23
9.73
What is the type of inhibition involved? [Select]
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