A biochemst wants to dtermine the effect of inhibitor on a certain enzyme. See attached data. Using linaer regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor  1. y- int ( up to 5 decimal places) 2. slope 3. Vmax 4. Km Using linear regression analysis determine the values of Vmax and Km of the enzyme in the presence of inhibitor 1. y- int ( up to 5 decimal places) 2. slope 3. Vmax 4. Km

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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A biochemst wants to dtermine the effect of inhibitor on a certain enzyme. See attached data.

Using linaer regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor 

1. y- int ( up to 5 decimal places)

2. slope

3. Vmax

4. Km

Using linear regression analysis determine the values of Vmax and Km of the enzyme in the presence of inhibitor

1. y- int ( up to 5 decimal places)

2. slope

3. Vmax

4. Km

[Substrate]
(mm)
5
10
20
50
75
V, (mm/min)
(without inhibitor)
1.6674
2.7182
3.9687
5.4818
5.9892
Vo(mm/min)
(with inhibitor)
1.4694
2.4492
3.6741
5.2493
5.8020
Transcribed Image Text:[Substrate] (mm) 5 10 20 50 75 V, (mm/min) (without inhibitor) 1.6674 2.7182 3.9687 5.4818 5.9892 Vo(mm/min) (with inhibitor) 1.4694 2.4492 3.6741 5.2493 5.8020
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