Vo (uM/min) Vo (uM/min) [Substrate] (mM) Control (no Inhibitor) +Inhibitor A (250 uM) 0.00 0.00 0.00 1.000 9.89 3.95 2.000 16.34 6.85 3.00 20.86 9.07 4.00 24.22 10.83 5.00 26.80 12.25 Control Inhibitor A X X X
Q: Name and explain in detail one pathological condition/state in which the activity of succinate…
A: The citric acid cycle and the respiratory electron transfer chain contain succinate dehydrogenase.…
Q: Bacteria and other microbes can be used to "clean up" an oil spill by breaking down oil into carbon…
A: Adenine, Guanine, Cytosine, and Thymine are the nitrogenous bases present in DNA. Adenine and…
Q: BSA Concentration (ug/mL) Abs 2000 2.25 1500 1.715 1000 0.915 750 1.156 500 0.714 250…
A: A standard curve is defined as a calibration curve that is used for quantitative estimations. In…
Q: Which of the following single-stranded DNA sequences is most likely to form a stem-loop structure?…
A: Double-stranded DNA consists of two polynucleotide chains each strand having a phosphodiester…
Q: Consider the complete oxidation of one mole of simple TAG containing behenic acid residues (22:0).…
A: TAG contains a glycerol backbone attached to three molecules of behenic acid through ester bonds. It…
Q: Use the allosteric constant (L) to explain how the Monod-Wyman- Changeux (MWC) model accounts for…
A: The Monod-Wyman-Changeux model (MWC model, also known as the symmetry model) in biochemistry…
Q: What is the net electric charge of a peptide that has the sequence and provide the one letter code…
A: The amino acids are composed of an amino group, a carboxyl group, a hydrogen atom, and a variable…
Q: Using good details, show how the function of two or three different nucleic acids is connected to…
A: Nucleic acids are chemical substances that occur naturally and act as the primary…
Q: Consider the fatty acids: (a) Arachidic acid (C20H40O2); molar mass = 312.5 g/mol) (b) Palmitoleic…
A: β-oxidation generally occurs in the mitochondria, and it is the pathway for degradation of fatty…
Q: The charging of a tRNA with an amino acid can be represented by the following equation:amino acid +…
A: Translation is a biochemical phenomena in which mRNA molecule gets translated to proteins. Charging…
Q: The peptide segment shown below is part of a right-handed alpha helix. Please indicate the hydrogen-…
A: Peptide bond is formed between two amino acids. Polymerisation of amino acids via peptide bond…
Q: what linkage does glycogen synthase catalyzes?
A: Glycogen synthase is an enzyme involved in glycogen synthesis. Glycogen is synthesized from extra…
Q: hat are the physiological effects of prostaglandins, explain how aspirin can block the synthesis of…
A: Introduction: Prostaglandins are a group of closely related biologically active lipids, that are…
Q: How many nucleotides would be required to synthesize a protein of 60 amino acids? 90 180…
A: Translation is the process by which ribosomes in the cytosol or endoplasmic inner membrane…
Q: Consider the complete oxidation of one mole of simple TAG containing arachidic acid residues (20:0).…
A: The fatty acids that are released in the digestion of triglycerides are broken down in a sequence of…
Q: Name of the vitamin source of CoASH A. B12 B. B3 C. B9 D.B1 E. B7
A: Some enzymes are dependent on cofactors for their enzymatic activity. There are two types of…
Q: Identify (1) the group where the lipid belongs, and (2) determine whether the lipid is saponifiable…
A: The given structure is of Cortisol also known as hydrocortisone. A saponifiable lipid is part of the…
Q: Choose from the options A.The structure of Zovirax is an analog of Thymine Guanine Adenine…
A: Introduction: Zovirax also known as Acyclovir is used to treat infections caused by a certain type…
Q: Identify three other methods commonly used to visualize lipids on a TLC plate. Specify the type or…
A: Thin-layer chromatography is a technique that is used for the separation and identification of…
Q: Which of the following statements is NOT true about Krebs Cycle? a. Krebs cycle is composed of…
A: Introduction: It is a series of enzyme-catalyzed reactions which is important in all living cells…
Q: 6. Explain briefly the importance of enzyme inhibition in biochemistry.
A: An enzyme is a protein that catalyzes biochemical reaction. It converts substrate into product. A…
Q: Calculate the unknown concentration of the PROTEIN B with an absorbance value of A358 given the…
A: Tp calculate the unknown concentration of protein B from absorbance value, we first need to plot a…
Q: Please state if the statements are true or false. 1. A pyranose is a sugar in Haworth projection…
A: The answer is provided below.
Q: The number of CO2 molecules liberated for each molecule of acetyl-CoA during oxidative…
A: The tricarboxylic acid cycle also called a citric acid cycle or Krebs cycle is the second stage of…
Q: II. ATP ACCOUNTING Provide what is being asked for. Show all relevant calculations and summarize…
A: 1 mole of lactose is hydrolyzed to produce 1 mole of glucose and 1 mole of galactose. Metabolism of…
Q: rearrange these in the correct order of events in an action potential.
A: The process of impulse transmission involves two stages known as resting membrane potential and…
Q: 1. You are working on an experiment with a newly discovered enzyme. This enzyme works most…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Branching decreases the solubility of glycogen. True False b. The branching enzyme forms the…
A:
Q: Please state if the statements are true or false. 1. An aplha 1,4 glycosidic linkage is considered…
A: The most common carbohydrates in diet are polysaccharides. They're polymeric carbohydrates with…
Q: Consider the complete oxidation of one mole of simple TAG containing lignoceric acid residues…
A: Fatty acids are degraded through the process of beta-oxidation in the mitochondrial matrix. Each…
Q: If there is an accumulation of acetyl CoA in the liver mitochondria, acetyl CoA is converted to…
A: Acetyl-CoA is formed from the end product of glycolysis i.e. pyruvate and then participates in TCA…
Q: Consider the complete oxidation of one mole of simple TAG containing behenic acid residues (22:0).…
A: Behenic acid is a saturated fatty acid with 22 carbon atoms and possesses the general formula,…
Q: Interaction of certain growth factors with their receptors leads to following sequence of events…
A: Mitogen-activated protein kinases (MAPK) are proteins that are serine/ threonine specific kinases…
Q: Consider the following figure when answering this question. Answer all of the questions on your own…
A: Monosaccharides are identified as one of the simplest carbohydrates. These compounds can not be…
Q: Which of the following statement(s) is/are TRUE about the digestion of fats in adults? I. The…
A: Fats are one of the nutrients in the balanced diet. True fats contain fatty acid molecules…
Q: 10 n 0 progress of reaction A graph of the free energy changes that take place during a reaction is…
A: Chemical reactions are classified into two types. Exergonic reactions that take place with the…
Q: Which statements are true? Explain why or why not.1 The chemical carcinogen…
A: Introduction: Cancer is the most common disease in the developing world and approximately one in…
Q: 1. make your own version of Urea Cycle with diagram. 2. explain why these cycles are important for…
A: Ammonia is the end product of protein metabolism. The urea cycle helps to remove the ammonia formed…
Q: A3 Glucosylacetamide has a structure similar to the transition state during catalysis by the enzyme…
A: The chemical compounds that have structure similar to that of transition state substrate are called…
Q: A heptapeptide was analyzed by Pinky. She reacted it with carboxypeptidase and found out that it…
A: A polypeptide is a sequence of amino acids that are linked to each other via peptide bonds. The bond…
Q: Label the x axis in each graph below with the appropriate factor (possible factors: temperature, pH,…
A: Introduction: Enzymes are proteins that function as biological catalysts to accelerate the rate of…
Q: Please answer fast Explain how you would go about developing new ribozymes capable of targeting new…
A: Ribozymes are the ones that arefound to be catalytically active RNA molecules. Here, RNA is…
Q: Which of the peptide sequences below best matches the hydropathy plot shown? 5 10 Residue Number 2…
A: Hydrophobicity is defined as the tendency of non-polar molecules to form aggregates to reduce their…
Q: 45. find the connection among the words below and choose the letter of the word which is different…
A: Gel electrophoresis is a technique used to identify, quantify, and separate nucleic acids. The…
Q: How do nuclear import receptorsnegotiate the tangled gel-like interior ofa nuclear pore complex so…
A: Nuclear pore complex is a large complex main up of protein, this complex allows small molecule and…
Q: 8. Which of the following is/are TRUE about bioassay? 1. It is a dose-response type of research. II.…
A: As a result of comparing a substance's effect on a test organism with a standard preparation, a…
Q: Using the figure below, what is molecule "A" (type a 1, 2 or 3 in the blank) nuclease ligase DNA…
A: DNA replication is the process of duplication of a DNA into two daughter DNAs. Each strand of the…
Q: Discuss briefly but concisely the importance of the following metabolic pathway. A. Glycolysis B.…
A: Different metabolic pathways occurs in the living cell like glycolysis, Krebs cycle, Electron…
Q: How many molecules of NADPH are needed to fix 1 molecule of carbon dioxide? Group of answer…
A: Carbon dioxide is fixed during the photosynthesis process. In photosynthesis, the carbon dioxide is…
Q: The enzyme responsible for the 1st committed step in the glycolytic pathway
A:
Find the control Vmax and Km value, and use this to find the Vmax apparent for inhibitor A.
Step by step
Solved in 3 steps with 2 images
- graph the data from the table after taking recipocals of [S], V without inhibitor and V with inhibitor. It should be Lineweaver - Burke. use graph paper or excel. however upload graph and answers as 1 document as jpeg, pdf, or word. there should be 2 lines on 1 graph and both lines should cross the Y axis and hit the X axis (negative X axis). label both axes, determine Km and Vmax (show work) in the presence and absence of inhibitor State type of inhibition and if inhibitor resembles the substrate or not. can more substrate relieve the inhibition? [S] (UM) 3 10 30 5 90 Velocity (umol minute-¹) No inhibitor 10.4 14.5 22.5 33.8 40.5 Inhibitor 4.1 6.4 11.3 22.6 33.8This is a walk-through of Minimum Inhibitory Concentration, MIC: This tray has 8 rows, 4 for Staphylococcus aureus (SA) and 4 for Methicillin-Resistant S. aureus (MRSA). Each row has 12 compartments, or wells. The wells have different concentrations (ug/ml) of the antibiotic Oxacillin in them. From Left to Right, the concentration of Oxacillin is going down acillin concentration, pg/ml 64 32 16 8 4 2 1 0.5 0.25 0.13 0.06 0 SA dn The same amount of S. aureus broth culture is added to each well and the tray is incubated. A color change shows that S. aureus grew in a given well. MRSA What is the lowest concentration at which SA is inhibited (does not grow)? 64mg/ml What is the lowest concentration at which MRSA is inhibited? What is the MIC for Oxacillin vs SA? What is the MIC for Oxacillin vs MRSA? - Notes Comments 33°FDetermine the Ki for the inhibitor at 30 °Cand decide what type of inhibitor is being used. Eo T I S V (g/I) (°C) (mmol/ml) (mmol/ml) (mmol/ml-min) 1.6 30 0.1 2.63 1.6 30 0.033 1.92 1.6 30 0.02 1.47 1.6 30 0.01 0.96 1.6 30 0.005 0.56 1.6 49.6 0.1 5.13 1.6 49.6 0.033 3.70 1.6 49.6 0.01 1.89 1.6 49.6 0.0067 1.43 1.6 49.6 0.005 1.11 0.92 30 0.1 1.64 0.92 30 0.02 0.90 0.92 30 0.01 0.58 0.92 30 0.6 0.1 1.33 0.92 30 0.6 0.033 0.80 0.92 30 0.6 0.02 0.57
- Why does a pure noncompetitive inhibitor not changethe observed KM?Consider the following experimental data from another experiment: [S] 1.5 2.00 2.50 5.00 10.00 V (No inhibitor) mmol ml¹ min¹ 0.167 0.204 0.232 0.313 0.385 V (inhibitor) mmol ml¹¹ min¹¹ 0.115 0.143 0.167 0.250 0.333 Calculate Km and V max and determine whether this inhibitor is competitive, non-competitive or uncompetitive.PTP1B Substrate kcat Km kcat/Km UM 10-7 x (s-1 M) DADEPYLIPQQG DADAPYLIPQQG DAAEPYLIPQQG AAAAPYLIPQQG 44.6 + 1.8 39.8 + 0.32 3.9 + 0.9 13.7 + 0.46 1.1 + 0.25 0.29 + 0.01 35.3 + 0.22 6.6 ± 0.22 0.53 + 0.02 34.7 + 0.25 52.7 ± 0.7 0.066 + 0.001 (d) (. ) The units for kcat/KM in the above are given according to standard scientific notation. On this basis what is the value of this kinetic parameter for the DADEPYLIPQQG substrate?
- What is DPI? What are the advantages of DPI over MDI? How drug is aerosolized from a DPI system? Please answer at your own words.PTP1B Substrate kcat Km. kcat/Km UM 10-7 x (s-1 M) DADEPYLIPQQG DADAPYLIPQQG DAAEP YLIPQQG AAAAPYLIPQQG 44.6 + 1.8 39.8 + 0.32 3.9 + 0.9 13.7 + 0.46 1.1 + 0.25 0.29 + 0.01 35.3 + 0.22 6.6 + 0.22 0.53 + 0.02 34.7 + 0.25 52.7 + 0.7 0.066 + 0.001 ) The units for kcat/KM in the above are given according to standard scientific notation. On this (d) ( basis what is the value of this kinetic parameter for the DADEPYLIPQQG substrate?A schematic representation of the enzyme IspD complexed to inhibitor 3, and a series of inhibitors 3-5 are shown below. Ala202 lle240 mwww NH NH Val263 ОН www HN N- lle177 HN 'N' CI 3 X = N 4 X = C-CN 5 X = C-COO IC50 274 µM IC50 140 nM IC50 35 nM NH2 HN Val266 N -N O-H---- N HN %3D Arg157 HN wwww lle265 Explain why structure 4 is a more potent inhibitor (lower IC50 value) than inhibitor 3 and why structure 5 is a much weaker inhibitor (higher IC50 value) than 3 and 4.
- 3. In this question you can do the graphs on your computer. A, Calculate the Vmax and Km in the presence and absence of the inhibitor. B. Determine what type of inhibitor has been added. C. Determine the rate in the absence of inhibitor when the substrate concentration is 100mM. [S]mM 2.3 3.2 4 8 10 1/[S] MM-1 0.44 0.31 0.25 0.125 0.1 SIN INHIBIDOR Vo μmol/sec 1/Vo sec/μmol 0.25 0.29 X 0.4 X 4 3.5 X 2.5 X CON INHIBIDOR Vo μmol/sec1/Vo sec/μmol X X 0.17 0.29 X X X 4 3.5 XExplain the relationship between the minimum inhibitory concentrationand the therapeutic index. What is optimum for each?Do not include zeros at the end of decimal numbers. The problems and drug orders are presented for practice only, and actual prescribed dosages factors. Order: Rocephin 500 mg IM Supply: Rocephin 1 gram Directions: Reconstitute with 2.1 mL of sterile water to yield 350 mg/mL Give: ML Moving to another question will save this response. Type here to search O E M 90 DELL