What is the impact of the lower value km 0.01 on the affinity of enzyme for substrate?
What is the impact of the lower value km 0.01 on the affinity of enzyme for substrate?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
What is the impact of the lower value km 0.01 on the affinity of enzyme for substrate?
![**Fig. 10: Michaelis-Menten Plot using a value of 0.01 mM for Km.**
Notice:
1) That a new set of calculated values in column C has been generated.
2) That by using these new data, a new best-fit Michaelis-Menten plot (red curve) has been generated.
Fig. 10 shows that the rates for the new enzyme-catalyzed reaction (red curve) were higher than those originally obtained (blue symbols).
**Detailed Explanation of Graphs and Diagrams:**
- **Table:**
- This includes columns labeled [S] (substrate concentration in mM), V0 (initial velocity), Km, and Vmax, with specific numerical values provided for each parameter. The new values for V0 are listed in column C.
- **Graph:**
- The bottom axes represent substrate concentration, and the vertical axes display reaction rate.
- The graph contains a red curve and blue symbols. The red curve represents the new Michaelis-Menten fit calculated with the new values, while the blue symbols represent previously obtained data points.
The visual illustrates the comparison between calculated values and initially obtained data, highlighting differences in enzyme activity based on altered parameters.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F5ef5f725-b08b-4f71-a80c-f37e47d07785%2F2020e9f9-f9c6-4116-aff7-76f2dc7b10f9%2Fgnp3ln_processed.jpeg&w=3840&q=75)
Transcribed Image Text:**Fig. 10: Michaelis-Menten Plot using a value of 0.01 mM for Km.**
Notice:
1) That a new set of calculated values in column C has been generated.
2) That by using these new data, a new best-fit Michaelis-Menten plot (red curve) has been generated.
Fig. 10 shows that the rates for the new enzyme-catalyzed reaction (red curve) were higher than those originally obtained (blue symbols).
**Detailed Explanation of Graphs and Diagrams:**
- **Table:**
- This includes columns labeled [S] (substrate concentration in mM), V0 (initial velocity), Km, and Vmax, with specific numerical values provided for each parameter. The new values for V0 are listed in column C.
- **Graph:**
- The bottom axes represent substrate concentration, and the vertical axes display reaction rate.
- The graph contains a red curve and blue symbols. The red curve represents the new Michaelis-Menten fit calculated with the new values, while the blue symbols represent previously obtained data points.
The visual illustrates the comparison between calculated values and initially obtained data, highlighting differences in enzyme activity based on altered parameters.
Expert Solution

This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps

Recommended textbooks for you

Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman

Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY

Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman

Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY

Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning

Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning

Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON