The enzymatic reaction rate r is given by equation 2 below as a function of substrate concentration Cs and kinetics parameters KM and rmax. Using linear and nonlinear regression in excel, evaluate the kinetic parameter constants of a biosystem given the gathered data from a series of batch runs

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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2. The enzymatic reaction rate r is given by equation 2 below as a function of substrate
concentration Cs and kinetics parameters KM and rmax. Using linear and nonlinear
regression in excel, evaluate the kinetic parameter constants of a biosystem given
the gathered data from a series of batch runs

Substrate Concentration
Initial Reaction Rate
Tmax Cs
r =
kM + Cs
(2)
mmol/L
mmol/L min
0.20
2
0.22
3
0.30
5
0.45
7
0.41
10
0.50
15
0.40
20
0.33
Transcribed Image Text:Substrate Concentration Initial Reaction Rate Tmax Cs r = kM + Cs (2) mmol/L mmol/L min 0.20 2 0.22 3 0.30 5 0.45 7 0.41 10 0.50 15 0.40 20 0.33
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