the maximal velocity of the reaction the Km value of the uninhibited reaction the Km value of the reaction with (i) the lower level of inhibitor and (ii) the higher level of the inhibitor.
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
The above graphs (Lineweaver-Burk) were plotted using data from an experiment that investigated changes in initial velocity, V0, (in mmol/min) as a function of substrate concentration, [S], (in mmol/L) for an enzyme-catalysed reaction. The initial velocity was measured at a fixed concentration of enzyme at different concentrations of substrate in the absence of any inhibitor and was then repeated in the presence of a competitive inhibitor at two different concentrations.
Use the graph to determine
- the maximal velocity of the reaction
- the Km value of the uninhibited reaction
- the Km value of the reaction with (i) the lower level of inhibitor and (ii) the higher level of the inhibitor.
TEMPLATE FOR ANSWERS
- Vmax of uninhibited reaction
Intercept on y-axis = = __________________
Vmax = __________________
- Km value of the uninhibited reaction
Intercept on x-axis = = __________________
= __________________
Km = __________________
- (i) Km value of the reaction at lower level of inhibitor
Intercept on x-axis = = __________________
= __________________
Km = __________________
(ii) Km value of the reaction at higher level of inhibitor
Intercept on x-axis = = __________________
= __________________
Km = __________________
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e above graphs (Lineweaver-Burk) were plotted using data from an experiment that
vestigated chanaes in initial velacity Va (in mmol/min) as a function of suhstrate
D. Focus](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Faebf0fbd-3f5b-4b76-9563-79708319335d%2Fb5ecc81b-c7b3-4ee1-9a60-b4ef596456c0%2Fj91pqxr_processed.jpeg&w=3840&q=75)
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