9. a. Which class of enzymes do poisonous snakes use to rock your world when they bite their prey? (3 pts) b. What do you think happens to the cells (red blood cells, specifically) after being bitten by an eastern diamondback rattler or the Indian cobra? c. These enzymes are also present in our body, albeit in a very low quantity. What purpose do these enzymes serve in our metabolic processes?

9. a. Which class of enzymes do poisonous snakes use to rock your world when they bite their prey? (3 pts) b. What do you think happens to the cells (red blood cells, specifically) after being bitten by an eastern diamondback rattler or the Indian cobra? c. These enzymes are also present in our body, albeit in a very low quantity. What purpose do these enzymes serve in our metabolic processes?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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4. Look up chymotrypsin and answer the following questions? a) Where in the body is this made? b) What type of enzyme is it?
A. How does each of the following irreversible inhibitors interact with the Enzymes? IV. 1. Cyanide 2. Heavy-metal ions B. List an antidote for each of these two poisons and describe how each functions.
1. 65 years old woman with low physical activity got the excess carbohydrates (about 400 g daily) for several years. The laboratory value of total cholesterol in serum blood was 260 mg/dl. Why the excess consumption of carbohydrates can cause hypercholesterolemia? To answer the question: a) compare the cholesterol concentration in the serum blood of the patient with the normal range; b) draw the schemes of the metabolic pathways confirming that overeating of carbohydrates can result in hypercholesterolemia; c) draw the scheme of the cholesterol synthesis regulation; d) give your prescriptions for the diet and treatment of the patient.
B) Read the situations below and indicate which of the four methods of enzyme regulation is occurring for each. a) The energy-carrying molecule ATP is made by the enzyme ATP synthase. Muscle cells use a lot of energy and also have higher amounts of the ATP synthase enzyme than many ouier cem types. General mechanism of enzyme regulation: /1 b) Prostaglandins are messenger molecules involved in the inflammatory response, as well as th perception of pain. They are synthesized from polyunsaturated fatty acid substrates by an enzyn called cyclo-oxygenase. "Ibuprofen" is the active ingredient in a variety of anti-inflammatory medications such as Motrin® and Advil®. It reduces pain and swelling by binding to a hydrophobic channel in the active site of cyclo-oxygenase, blocking the polyunsaturated fatty acids from binding to the enzyme, and therefore stopping production of prostaglandins. General mechanism of enzyme regulation: a) In point form, describe the steps by which ATP is produced…
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3) Read the situations below and indicate which of the four methods of enzyme regulation is occurring for each. a) The energy-carrying molecule ATP is made by the enzyme ATP synthase. Muscle cells use a lot of energy and also have higher amounts of the ATP synthase enzyme than many other cell types. General mechanism of enzyme regulation: S b) Prostaglandins are messenger molecules involved in the inflammatory response, as well as the perception of pain. They are synthesized from polyunsaturated fatty acid substrates by an enzym called cyclo-oxygenase. "Ibuprofen" is the active ingredient in a variety of anti-inflammatory medications such as Motrin® and Advil®. It reduces pain and swelling by binding to a hydrophobic channel in the active site of cyclo-oxygenase, blocking the polyunsaturated fatty acids from binding to the enzyme, and therefore stopping production of prostaglandins. General mechanism of enzyme regulation:
One condition is ketoacidosis (low blood pH) which is a potentially life-threatening state, most commonly experienced by diabetics. Ketoacidosis, however, is not typically an issue for healthy people who are following a ketogenic diet. What is the difference between ketoacidosis and ketosis? Why do diabetics sometimes develop ketoacidosis, while healthy people rarely do? Healthy people are well equipped to survive on a ketogenic diet. Why is it important that humans evolved to have the ability to survive without carbohydrate nutrients?
In the 1930's a chemical known as DNP was touted as en effective diet pill. Weight loss was dramatic in patients taking this drug. However, to quote Efraim Racker (A New Look at Mechanisms in Bioenergetics, Academic Press, 1976, p. 155), ..."the treatment eliminated not only the fat but also the patients,... This discouraged physicians for awhile..." This drug promotes weight loss by causing the leakage of H* across the inner mitochondrial membrane. Which of the following would you expect to see in patients taking DNP? [Select the best answer.] decreased ability to produce ATP increased ability to produce ATP decreased ability to use the Citric Acid Cycle to produce ATP slowing down the Citric Acid Cycle.
On the right is a diagram of plasma concentration of three metabolites: glucose, B- hydroxybutyrate, and free fatty acid of an experimental animal over weeks of starvation. 4. (a) Label the lines with the names of the three metabolites. Yellow: Red: 10 20 30 40 Blue: Days of starvation (b) What factor in the plasma binds to and transports free fatty acid (c) Where was B-hydroxybutyrate produced? Why it is produced? What are the purposes of producing it (d) How B-hydroxybutyrate is used as fuel in extrahepatic cells? Write down the reactions, enzymes, and cofactors (No need to elaborate on the TCA cycle). (e) How many ATP molecules or equivalent are generated after complete oxidation of one molecule of B-hydroxybutyrate? Show your calculations. Hint: You need to consider ATP consumption for bypassed steps that may normally yield or consume AT Plasma concentration (mM)
2.A person did not get food for 3 days and had lost his weight, ketone bodies were revealed in urine. To explain this: a)describe the main metabolic pathways providing energy for the liver cells during starvation and explain its regulation; b) discuss the hormonal changes; c)predict the deleterious effect of prolonged starvation.
4. A sportsman is doing a 10 km marathon for 1.5 hours. What changes in lipid metabolism in adipose tissue and muscles are triggered by long-term physical activity? To answer the question: a) name the hormone, controlling the lipid metabolism in adipocytes and write down the scheme of the process; b) explain, how some of the products of lipid mobilization, transported from adipocytes to the bloodstream, would be utilized by skeletal muscles and liver in these conditions; c) calculate the ATP yicld in the result of palmitolcic acid oxidation.
1. Pepsinogen (an inactive form of the enzyme) that forms in the main cells of the stomach has a molecular weight of 42,000 D. In the gastric juice, the pepsinogen turns into the active enzyme pepsin, while its molecular weight decreases to 35,000 D. Explain the mechanism of regulation of enzyme activity. To do this: a) draw a scheme for regulating the activation of pepsin; b) name the class of the enzyme that converts pepsinogen to pepsin; c) indicate which levels of protein structural organization change when pepsin is activated.