8) You perform flow cytometry on a population of cells treated with a mutagen to identify new factors required for cell cycle progression. You isolate two new mutants that display a change in the rate of the cell cycle. You measure DNA content with flow cytometry from a control cell population as well as from these two mutants. The results from this experiment are shown below.
8) You perform flow cytometry on a population of cells treated with a mutagen to identify new factors required for cell cycle progression. You isolate two new mutants that display a change in the rate of the cell cycle. You measure DNA content with flow cytometry from a control cell population as well as from these two mutants. The results from this experiment are shown below.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:8) You perform flow cytometry on a population of cells treated with a mutagen
to identify new factors required for cell cycle progression. You isolate two
new mutants that display a change in the rate of the cell cycle. You measure
DNA content with flow cytometry from a control cell population as well as
from these two mutants. The results from this experiment are shown below.
Control
Mutant 1
Mutant 2
1
Relative amount of DNA per cell
Use these data to answer the following questions.
1) If you measured levels of M-cyclin protein from these different
populations, which would have the HIGHEST.
a. Control
b. Mutant 1
c. Mutant 2
2) After genome sequencing, you identify a loss-of-function mutation in
Cdc20 in one of these cell populations. Based on these data, which
population would you expect to have this mutation?
a. Mutant 1
b. Mutant 2
3) If DNA damage is sensed during replication and cell cycle
progression is stopped, which of the graphs would you expect to
observe?
a. Control
b. Mutant 1
c. Mutant 2
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