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A B cat frame-shift no cat no cat cat nonsense cat C % survival 120 100 80 60 40 20 0 42 cat cats cats 37 30 cats 23 [°C]

A B cat frame-shift no cat no cat cat nonsense cat C % survival 120 100 80 60 40 20 0 42 cat cats cats 37 30 cats 23 [°C]

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Explain the technique used in the figure of each panel and what the data shows for each. 

  Panel Technique These data show:
Figure 4

A

B

c

    
Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Panel A B C A B C D E A B C D A B C Technique figure 1A-fluorescent microscopy was used These data show: figure 1A-low level GFP protein expression was observed in B. subtilis mutants with frameshift and nonsense mutations in the GFP gene
Transcribed Image Text:Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Panel A B C A B C D E A B C D A B C Technique figure 1A-fluorescent microscopy was used These data show: figure 1A-low level GFP protein expression was observed in B. subtilis mutants with frameshift and nonsense mutations in the GFP gene
A B cat frame-shift no cat no cat cat nonsense cat C 120 100 80 60 40 20 % survival 0 42 catt cats cats 37 30 cats 23 [°C] Fig. 4. Errors in gene expression enable survival of strains carrying a mutated antibiotic resistant gene. (A) Strains PY79 (no cat), SB463 (cat), SB477 (catfs), and SB475 (catns) were grown on 4-µg/mL chloramphenicol-containing plates. Plates were imaged after 40 h of incubation at 30 °C. (B) Strains PY79 (no cat), SB463 (cat), SB477 (catf), and SB475 (catns) were streaked on 10-µg/mL chloramphenicol- containing plates and imaged after 40 h of incubation at 30 °C. (C) The viability (%) of SB477 (catfs) (red bars) and SB475 (catns) (blue bars) at different temperatures when plated on chloramphenicol-containing plates. Cells were grown in CH medium at the different temperatures in the absence of chloramphenicol, and plated at mid-exponential phase on either LB or LB supplemented with 4-µg/mL chloramphenicol plates. Percent-viability is the number of colonies growing on chloramphenicol-containing plates relative to the total number of colonies growing on LB plates incubated at the equivalent temperature (Materials and Methods). PY79 (no cat) strain did not grow on chloramphenicol-containing plates, whereas SB463 (cat) showed ~100% viability at any growth temperature. The viability on LB plates of all tested strains was similar, with colony number ranging from 350 to 500.
Transcribed Image Text:A B cat frame-shift no cat no cat cat nonsense cat C 120 100 80 60 40 20 % survival 0 42 catt cats cats 37 30 cats 23 [°C] Fig. 4. Errors in gene expression enable survival of strains carrying a mutated antibiotic resistant gene. (A) Strains PY79 (no cat), SB463 (cat), SB477 (catfs), and SB475 (catns) were grown on 4-µg/mL chloramphenicol-containing plates. Plates were imaged after 40 h of incubation at 30 °C. (B) Strains PY79 (no cat), SB463 (cat), SB477 (catf), and SB475 (catns) were streaked on 10-µg/mL chloramphenicol- containing plates and imaged after 40 h of incubation at 30 °C. (C) The viability (%) of SB477 (catfs) (red bars) and SB475 (catns) (blue bars) at different temperatures when plated on chloramphenicol-containing plates. Cells were grown in CH medium at the different temperatures in the absence of chloramphenicol, and plated at mid-exponential phase on either LB or LB supplemented with 4-µg/mL chloramphenicol plates. Percent-viability is the number of colonies growing on chloramphenicol-containing plates relative to the total number of colonies growing on LB plates incubated at the equivalent temperature (Materials and Methods). PY79 (no cat) strain did not grow on chloramphenicol-containing plates, whereas SB463 (cat) showed ~100% viability at any growth temperature. The viability on LB plates of all tested strains was similar, with colony number ranging from 350 to 500.
Expert Solution
Step 1

Fig 4:

Panel A-

Technique: Fluorescence microscopy has been used. All the 4 strains  are grown on in chloramphenicol containing plates.

Data shows- The GFP protein expression is seen in the SB 463 (cat) , frameshift SB 477catfs  and nonsense mutants SB475 catns.

 

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