BglII 6. You have a circular plasmid available, with a single RE site for BglII (A^GATCT). You cleave the plasmid with BglII, and join it to both ends of a linear fragment of viral DNA which was obtained by digesting the viral genome with the RE DpnII (^GATC), resulting in a larger plasmid. ☑ DpnII Text DpnII O If the resulting larger plasmid is incubated with BglII, will the enzyme be able to excise the fragment of viral DNA? If no, why not? If yes, explain under which conditions. Motivate your answer by showing the sequence of the plasmid DNA (in black) and the viral DNA (in blue) at both the join positions. Write out the sequences in dsDNA format, if necessary indicate any flanking nucleotides by N. 7. Consider the following plasmid (size 6700 bp), with restriction sites at the positions indicated: BamHI + 1 6700 bp 2800 3500 EcoRI BamHI Probe

BglII 6. You have a circular plasmid available, with a single RE site for BglII (A^GATCT). You cleave the plasmid with BglII, and join it to both ends of a linear fragment of viral DNA which was obtained by digesting the viral genome with the RE DpnII (^GATC), resulting in a larger plasmid. ☑ DpnII Text DpnII O If the resulting larger plasmid is incubated with BglII, will the enzyme be able to excise the fragment of viral DNA? If no, why not? If yes, explain under which conditions. Motivate your answer by showing the sequence of the plasmid DNA (in black) and the viral DNA (in blue) at both the join positions. Write out the sequences in dsDNA format, if necessary indicate any flanking nucleotides by N. 7. Consider the following plasmid (size 6700 bp), with restriction sites at the positions indicated: BamHI + 1 6700 bp 2800 3500 EcoRI BamHI Probe

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section: Chapter Questions

Problem 3TYK: Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning...

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Transcribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’
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