A bacterial culture is initially composed of 100 cells. After 1 hour the number of bacteria is 1.5 times the initial population.a. If the rate of growth is proportional to the number of bacteria present, determine the time necessary for the number ofbacteria to triple.b. What is the time required for a culture with 1x106 of the same bacteria to triple? Explain your results.c. Under what conditions would the answers obtained in (b) be invalid?

The given answer calculates how long it will take for bacterial populations to quadruple in perfect…

Answered: A bacterial culture is initially…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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You have a culture of cells at 3.5 x 108 cells/ml and you want to prepare a fresh 5 ml culture with a starting density of 1 x 104 cells/ml. What volume of the old culture do you add to the new 5 ml culture?
Your first task in the lab is to learn how to culture these cells and begin expanding the culture (increasing the # of cells) so that you may later conduct experiments on them. You take two aliquots, each with an equal number of cells, and place them in two different culture vessels, one is a sealed airtight flask (culture 1) and the other is an open topped culture dish (culture 2). You provide both with the same growth media that contains their preferred fuel source, glucose. Will one of these cultures (#1 or #2 as described above) need to have their media changed more frequently than the other? (Yes or No) If yes, which culture will need to have its media changed more often and why? If no, why not?
A medium was inoculated with 5 * 106 cells/ml of Escherichiacoli cells. Following a 1-h lag, the population grewexponentially for 5 h, after which the population was 5.4 * 109cells/ml. Calculate g and k for this growth experiment.
Explain how to conduct a Kirby-Bauer disk diffusion test. Include all of the steps involved.
The culture you are working has a doubling time of 2 hours and a cell density of 3 x 106 cells per mL. For your experiment, you first dilute the culture 100 fold. Assuming that there is no lab phase and that the cells remain in exponential growth the entire time, what is the cell density (cells/mL) after 10 hours?
All of the following are valid measurements of growth except: A. Counting viable cells B. Estimating the weight of cells C. Determining the dry weight of cells D. Streaking for single colonies E. Measuring some cellular component F. Counting the total number of cells
If the volume of a Staphylococcus aureus cell is estimated at 0.5 μm3, how many cells could be accommodated, in principle, in 5 mL of saturated culture? (1 mL = 1 cm3). Show your calculations.
). Consider a culture medium on which only gram-positive organisms such as Staphylococcus aureus colonies can grow due to an elevated NaCl level. A yellow halo surrounds the growth, indicating the bacterium fermented sugar in the medium, decreasing the pH as a result and changing the color of a pH indicator chemical. This type of medium would be referred to as a(n): A) Selective medium. B) Differential medium. C) Enrichment culture. D) Selective and differential medium. E) Differential and enrichment culture.
In an experiment to calculate the decimal reduction time for an Escherichia coli culture, viable cells were exposed to a constant temperature of 80°C for a set amount of time. After exposure, the remaining number of surviving cells were counted. Based on Table 1, what is the decimal reduction time?Table 1. Decimal Reduction Time for E. coli Heated to 80°C Total time of exposure (minutes): Number of Microbial Cells Present: 0 100 1 80 3 50 4 42 6.5 26 13 10 21 0
B) A total of 2.5 x 105 cells were harvested from the patient. You need the cells to fill a scaffold with dimensions of 20mm x 20mm x 4mm at a final density of 2 x 104 cells/mm³. After culturing the harvested cells, you found that they have a doubling time of 36 hours. Determine the total number of cells (N) needed to fill the scaffold. Show your work.
Use the following passage to answer 2 consecutive questions. A urine specimen was collected in a container and placed on the central desk for delivery at 10:00 am. The specimen is not sent to the lab until 5 hours later (3:00 pm). The sample time was marked by the central clerk as collected at 4:00 pm. The bacterial species has a generation time of 30 minutes at room temperature. There are 100 bacterial cells/ml at the time of collection (10:00 am). The laboratory will cite a bacterial infection at more than 1000 bacterial cells/ml.
How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume? suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10^-1 dilution of the entire culture. Explain how you would do this. Show your calculations.

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