5 Why is it important to measure the masses of the NaCl and KNO3 samples in Part B? 5 Why is it important to measure the masses of the NaCl and KNO3 samples in Part B?
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- 5. Anesthetic gases used in surgery are known to bind to the hemoglobin molecule in red blood cells. The diagram below illustrates O2 binding curves of normal human HbA in the presence of the anesthetic gas dichloromethane (DCM). O UNTREATED 100 Symbols: a Dсм 23 Torr • DCM 50 Torr x DCM I00 Torr o, 0 Torr DCM; О, 23 Torr DСМ Д, 50 Torr DСМ x, 100 Torr DCM. 80 60 40 (1 Torr = 1 mm Hg.) 20 The solutions were buffered to pH 7.4. 0.5 1.0 1.5 2.0 log p02 For the curves at 0 and 100 Torr of dichloromethane, draw their equivalent in the form of (a) (* Hill plots. Label the axes and indicate on both the plot above and the Hill plot where the value of the dissociation equilibrium constant Kd for O2 binding is defined for 0 and 100 Torr of dichloromethane Kd Hb(O2)n nO2 Hb + Be sure to label the axes, indicate the units on both axes, and make the Hill plot so that the values of Kd are equivalent to those on the saturation plot above. Indicate whether cooperativity, as indicated by the Hill…7. 8. 7. These oval structures are They are connected by #8, tubes called The fluid inside the tubes is called 96. Of the following pairs write which ones could be used as buffers and why: (a) 0.1 M NaOH and 0.1 M NaCH3COO, (b) 0.01 M H2SO4 and 0.01 M K2S04, (c) 0.01 M NH3 and 0.02 M NH4CI, (d) 0.2 M HNO2 and 0.2 M NaNO2.
- 5. We have 100 mL of 5X TBE (Tris/Borate/EDTA) solution and need to make 1 L of 2X solution. Solid compounds also exist for each of these materials. The molecular weight of Tris is 157.60; Borate has molecular weight of 58.80 and EDTA has a molecular weight of 292.44. a) What is the stock concentration? b) What is the final concentration? c) What is the starting volume? d) What is the final volume? e) Write out the equation used to solve this. Show all your work. Are we able to make a solution using this dilution method using are starting materials? If not, propose another way to make the desired concentration and volume of the solution. Show your work.5. Anesthetic gases used in surgery are known to bind to the hemoglobin molecule in red blood cells. The diagram below illustrates O2 binding curves of normal human HbA in the presence of the anesthetic gas dichloromethane (DCM). O UNTREATED 100 a DCM 23 Tor • DCM 50 Torr x DCM 100 Torr Symbols: o, 0 Torr DCM; O, 23 Torr DCM A, 50 Torr DCM x, 100 Torr DCM. 80 40 (1 Torr = 1 mm Hg.) The solutions buffered to pH 7.4. 20 were 0.5 1.0 1.5 2.0 log po2 % OXYGENATION6. What volume of a 9% NaCl solution is needed to prepare 100 mL of a 0.9% NaCl solution?
- 5. Anesthetic gases used in surgery are known to bind to the hemoglobin molecule in red blood cells. The diagram below illustrates O2 binding curves of normal human HbA in the presence of the anesthetic gas dichloromethane (DCM). O UNTREATED 100 a Dсм 23 Torr Symbols: в Dсм 50 Torr x DCM I00 Torr o, 0 Torr DCM; O, 23 Torr DCM A, 50 Torr DCM x, 100 Torr DCM. 80 60 40 (1 Torr = 1 mm Hg.) 20 The solutions were buffered to pH 7.4. 0.5 1.0 1.5 2.0 log p02 (a) (* Hill plots. Label the axes and indicate on both the plot above and the Hill plot where the value of the dissociation equilibrium constant Ka for O2 binding is defined for 0 and 100 Torr of dichloromethane For the curves at 0 and 100 Torr of dichloromethane, draw their equivalent in the form of Kd Hb(O2)n Hb + nO2 % OXYGENATION115. a) Describe the Biological Mass Transport Process b) Describe Henry's Law for Phase Equilibrium
- 3b. Second, you will make 10ml of 200µg/ml (Hint: Use CiVi=C2V2) How much 1000ug /ml BSA will you add to make 10mL of 200µg/ml? How much water will you add to this solution?8. What mass of solute is needed to prepare each of 1.00 L of 0.125 M K2SO4 solutions? (2 Points) 21.8 g K2SO4 218.0 g K2SO4 2.816 g K2SO4 28.16 g K2SO4o NB4: Why do you think you first had to make the 1 mM solution instead of working directly from the stock solution?