The role of the Histidine above the binding site in hemoglobin has been investigated by site-directed mutagenesis. For example, this Histidine has been replaced by the amino acid alanine which has a methyl group (R-CH3) instead of the histidine residue.(a) Would you expect the ability of hemoglobin to bind O2 to be affected by this change? (b) Would you expect the ability of hemoglobin to bind CO (carbon monoxide) to be affected by this change?

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The role of the Histidine above the binding site in hemoglobin has been investigated by site-directed mutagenesis. For example, this Histidine has been replaced by the amino acid alanine which has a methyl group (R-CH3) instead of the histidine residue.
(a) Would you expect the ability of hemoglobin to bind O2 to be affected by this change? 
(b) Would you expect the ability of hemoglobin to bind CO (carbon monoxide) to be affected by this change?

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