Explain why you did not obtain 100% recovery of GFP.   Almost all of the GFP came off in the 3 saved fractions. GFP was denatured on the column. ( Incorrrect)  GFP was not loaded onto the column. GFP was not purified by the column. GFP came off in many more fractions than we saved as we were looking for purity, not quantity.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter6: Basic Clinical Chemistry
Section6.5: Blood Glucose And Hemoglobin A1c
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Explain why you did not obtain 100% recovery of GFP.
 
Almost all of the GFP came off in the 3 saved fractions.
GFP was denatured on the column. ( Incorrrect) 
GFP was not loaded onto the column.
GFP was not purified by the column.
GFP came off in many more fractions than we saved as we were looking for purity, not quantity.
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