400 bp Hae III 380 bp Bam HI Eco RI 150 bp Hind II Plasmid DNA 2000 bp 250 bp 500 bp Eco RI 320 bp Eco RV a. Look up the restriction enzyme and indicate whether the restriction enzyme will produce a blunt or staggered cut. (1 mark) Note: Type either Blunt or Staggered Restriction enzyme Type of cut (Blunt vs Staggered) Eco RI b. Based on the plasmid map, list the size of fragments produced when the following restriction enzymes are used. Note: to answer correctly you must order the fragments from smallest to largest and separate each with a comma. There should be no spaces between numbers and commas. e.g. 400,500,530 Restriction Enzyme Eco RI and Hind II Eco RV and Bam HI Hae III, Eco RI and Bam HI Fragment size

400 bp Hae III 380 bp Bam HI Eco RI 150 bp Hind II Plasmid DNA 2000 bp 250 bp 500 bp Eco RI 320 bp Eco RV a. Look up the restriction enzyme and indicate whether the restriction enzyme will produce a blunt or staggered cut. (1 mark) Note: Type either Blunt or Staggered Restriction enzyme Type of cut (Blunt vs Staggered) Eco RI b. Based on the plasmid map, list the size of fragments produced when the following restriction enzymes are used. Note: to answer correctly you must order the fragments from smallest to largest and separate each with a comma. There should be no spaces between numbers and commas. e.g. 400,500,530 Restriction Enzyme Eco RI and Hind II Eco RV and Bam HI Hae III, Eco RI and Bam HI Fragment size

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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