Estimate the values for Vmax and Km. Vmax = μM/min Km = μM A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (UO) at different substrate concentrations ([S]). First, move the line labeled Vmax to a position that represents the maximum velocity of the enzyme. Next, move the line labeled 1/2 Vmax to its correct position. Then, move the line labeled Km to its correct position. Estimate the values for V max and Km. 1/2 V max Michaelis-Menten curve 300 V max 275 250 225 200 v (μM/min) 175 150 K 2 125 m 100 75 50 25 0 0 10 20 20 50 58 30 40 50 60 60 [S] (μM) 70 10 60 80 90 100

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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Estimate the values for Vmax and Km.
Vmax
=
μM/min
Km
=
μM
Transcribed Image Text:Estimate the values for Vmax and Km. Vmax = μM/min Km = μM
A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (UO) at different substrate concentrations ([S]).
First, move the line labeled Vmax to a position that represents
the maximum velocity of the enzyme.
Next, move the line labeled 1/2 Vmax to its correct position.
Then, move the line labeled Km to its correct position.
Estimate the values for V max and Km.
1/2 V
max
Michaelis-Menten curve
300
V
max
275
250
225
200
v (μM/min)
175
150
K
2 125
m
100
75
50
25
0
0
10
20
20
50
58
30
40
50
60
60
[S] (μM)
70
10
60
80
90
100
Transcribed Image Text:A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (UO) at different substrate concentrations ([S]). First, move the line labeled Vmax to a position that represents the maximum velocity of the enzyme. Next, move the line labeled 1/2 Vmax to its correct position. Then, move the line labeled Km to its correct position. Estimate the values for V max and Km. 1/2 V max Michaelis-Menten curve 300 V max 275 250 225 200 v (μM/min) 175 150 K 2 125 m 100 75 50 25 0 0 10 20 20 50 58 30 40 50 60 60 [S] (μM) 70 10 60 80 90 100
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