4.08 H H ↓ 1.02 ↓ | 2.54 11.02↑ E E Note: 1.67 = EXON = INTRON E 10.8 kbp 3.94 3.66 E = EcoRI site H = Hindill site H 10.811 E E 1.76 1.10 Fragment sizes are not to scale and all fragment lengths are in kilobase pairs (kbp) a. Indicate what enzymes you would use for digestion and the gel electrophoresis pattern of the restriction fragments (draw a diagram of the gel with fragment positions, indicate field polarity, and direction of migration). Please do single and a double digest of the DNA.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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4.08
2.54
H H
1.02
1.02 1.67
E E
= EXON
= INTRON
E
10.8 kbp
3.94
3.66
E = EcoRI site
wwwwwwwww
H = Hindill site
HI
Н
10.31t
E E
1.76
1.10
Note: Fragment sizes are not to scale and all fragment
lengths are in kilobase pairs (kbp)
The
a.
Indicate what enzymes you would use for digestion and the gel
electrophoresis pattern of the restriction fragments (draw a diagram of the gel
with fragment positions, indicate field polarity, and direction of migration).
Please do single and a double digest of the DNA.
Grammarly
Transcribed Image Text:4.08 2.54 H H 1.02 1.02 1.67 E E = EXON = INTRON E 10.8 kbp 3.94 3.66 E = EcoRI site wwwwwwwww H = Hindill site HI Н 10.31t E E 1.76 1.10 Note: Fragment sizes are not to scale and all fragment lengths are in kilobase pairs (kbp) The a. Indicate what enzymes you would use for digestion and the gel electrophoresis pattern of the restriction fragments (draw a diagram of the gel with fragment positions, indicate field polarity, and direction of migration). Please do single and a double digest of the DNA. Grammarly
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