Where would you find the base pairs that constitute the promoter? h. Which intron interrupts the 3′ UTR? i. Where would you find the sequences encoding the C terminus?
. The human gene for ß2 lens crystallin has the components listed below. The numbers represent nucleotide
pairs that make up the particular component. Assume
for simplicity that no alternative splicing is involved.
5′ UTR 174
1st exon 119
1st intron 532
2nd exon 337
2nd intron 1431
3rd exon 208
3rd intron 380
4th exon 444
4th intron 99
5th exon 546
3′ UTR 715
Answer the following questions about the ß2 lens
crystallin gene, primary transcript, and gene product.
Questions asking where should be answered with one
of the 11 components from the list or with None.
Assume poly-A tails contain 150 As.
a. How large is the ß2 lens crystallin gene in bp (base
pairs)?
b. How large is the primary transcript for ß2 lens
crystallin in bases?
c. How large is the mature mRNA for ß2 lens crystallin in bases?
d. Where would you find the base pairs encoding the
initiation codon?
e. Where would you find the base pairs encoding the
stop codon?
f. Where would you find the base pairs encoding the
5′ cap?
g. Where would you find the base pairs that constitute
the promoter?
h. Which intron interrupts the 3′ UTR?
i. Where would you find the sequences encoding the
C terminus?
j. Where would you find the sequence encoding the
poly-A tail?
k. How large is the coding region of the gene in bp
(base pairs)?
l. How many amino acids are in the ß2 lens crystallin
protein?
m.Which intron interrupts a codon?
n. Which intron is located between codons?
o. Where would you be likely to find the site specifying
poly-A addition?
question g h i
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
