3.A newly discovered gene has the following structure:4.08H H1.02| 2.54 ↑1.02↑E E1.67= EXON= INTRONE10.8 kbp3.943.66E = EcoRI siteVARMAANH = Hindill siteHI10.811E E1.761.10Note: Fragment sizes are not to scale and all fragmentlengths are in kilobase pairs (kbp)1Describe how you can determine if the gene is interrupted and, if so, thenumber of interruptions by restriction endonuclease analysis and Southernanalysis.a.Indicate what enzymes you would use for digestion and the gelelectrophoresis pattern of the restriction fragments (draw a diagram of the gelwith fragment positions, indicate field polarity, and direction of migration).Please do single and a double digest of the DNA.b. Draw the appearance of the autoradiogram of the Southern blot afterhybridization with a cDNA probe.

Name: 3.A newly discovered gene has the following structure:4.08H H1.02| 2.
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Description: 3.A newly discovered gene has the following structure:4.08H H1.02| 2.54 ↑1.02↑E E1.67= EXON= INTRONE10.8 kbp3.943.66E = EcoRI siteVARMAANH = Hindill siteHI10.811E E1.761.10Note: Fragment sizes are not to scale and all fragmentlengths are in kilobase

(a) A method used frequently in labs to separate charged molecules like DNA, RNA, and proteins is…

Answered: A newly discovered gene has th 4.08…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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3.
A newly discovered gene has the following structure:
4.08
H H
1.02
| 2.54 ↑1.02↑
E E
1.67
= EXON
= INTRON
E
10.8 kbp
3.94
3.66
E = EcoRI site
VARMAAN
H = Hindill site
HI
10.811
E E
1.76
1.10
Note: Fragment sizes are not to scale and all fragment
lengths are in kilobase pairs (kbp)
1
Describe how you can determine if the gene is interrupted and, if so, the
number of interruptions by restriction endonuclease analysis and Southern
analysis.
a.
Indicate what enzymes you would use for digestion and the gel
electrophoresis pattern of the restriction fragments (draw a diagram of the gel
with fragment positions, indicate field polarity, and direction of migration).
Please do single and a double digest of the DNA.
b. Draw the appearance of the autoradiogram of the Southern blot after
hybridization with a cDNA probe.

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Describe how you can determine if the gene is interrupted, and if so the number of interruptions by restriction endonuclesse analysis and southern analysis?
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