4. The graph below was taken from a paper by Galardi et al., 2007, studying the role of two microRNAs (mIR-221 and mIR-222) in prostate cancer progression. The researchers identified that these cancerous cells have an upregulation in both mlR221 and miR 222. Use the graphs below to answer the questions A-B. Background: Panel A shows the nucleotide sequence of both miRs and a cell cycle inhibitor gene known as p27. Panel B shows protein levels of the p27 gene in three different cell lines, PC3 (a fast- growing cancer) and 22RV1 and LNCAP (two slow-growing cancers). A miR-221 3-CUUUGGGUCGUCUGUUACAUCGA-5" ::: I : IIIIIII 1320-AUAGUUUUUACCUUUUAUGUAGCA -1: p27 Control miR-222 3-UCUGGGUC-AUCGGUCUACAUCGA-5" - P27 knock down В p27 10 actin --- 48 Sivia Galardi et al. J. Biol. Chem. 2007-28223716-23724 JBC time (h) Answer the following question(s): A. Summarize what you think the relationship between miR-221 and miR-222 with p27 in relation to the progression of cancer. PC3 22RV1 LNCAP cell number (x1000)
4. The graph below was taken from a paper by Galardi et al., 2007, studying the role of two microRNAs (mIR-221 and mIR-222) in prostate cancer progression. The researchers identified that these cancerous cells have an upregulation in both mlR221 and miR 222. Use the graphs below to answer the questions A-B. Background: Panel A shows the nucleotide sequence of both miRs and a cell cycle inhibitor gene known as p27. Panel B shows protein levels of the p27 gene in three different cell lines, PC3 (a fast- growing cancer) and 22RV1 and LNCAP (two slow-growing cancers). A miR-221 3-CUUUGGGUCGUCUGUUACAUCGA-5" ::: I : IIIIIII 1320-AUAGUUUUUACCUUUUAUGUAGCA -1: p27 Control miR-222 3-UCUGGGUC-AUCGGUCUACAUCGA-5" - P27 knock down В p27 10 actin --- 48 Sivia Galardi et al. J. Biol. Chem. 2007-28223716-23724 JBC time (h) Answer the following question(s): A. Summarize what you think the relationship between miR-221 and miR-222 with p27 in relation to the progression of cancer. PC3 22RV1 LNCAP cell number (x1000)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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Question
Transcribed Image Text:4. The graph below was taken from a paper by Galardi et al., 2007, studying the
role of two microRNAs (mIR-221 and mIR-222) in prostate cancer progression.
The researchers identified that these cancerous cells have an upregulation in
both mIR221 and miR 222. Use the graphs below to answer the questions A-B.
Background: Panel A shows the nucleotide sequence of both miRs and a cell cycle
inhibitor gene known as p27.
Panel B shows protein levels of the p27 gene in three different cell lines, PC3 (a fast-
growing cancer) and 22RV1 and LNCAP (two slow-growing cancers).
A miR-221
3'-CUUUGGGUCGUCUGUUACAUCGA-5
::: I : IIIII
1320-AUAGUUUUUACCUUUUAUGUAGCA -1:
р27
Control
miR-222
3-UCUGGGUC-AUCGGUCUACAUCGA-5
35
e P27 knock
30
down
В
p27
actin
48
Silvia Galardi et al. J. Biol. Chem. 2007:282-23716-23724
JBC
time (h)
Answer the following question(s):
A. Summarize what you think the relationship between miR-221 and miR-222 with
p27 in relation to the progression of cancer.
B. If you were to develop a drug, how would you manipulate levels of miRs and
p27?
PC3
22RV1
LNCAP
cell number (x1000)
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