35.Draw a bacterial gene (dsDNA and mRNA) showing the promoter (-35 and -10 consensus sense strand sequences), the TC start site (TSS), start of TL, open reading frame (ORF), end of TL, TC termination site (TTS), 5’-UTR, and 3’-UTR. Some of these landmarks are on the dsDNA, some on the mRNA. The TSS and TTS can be drawn on both. Indicate 5’ and 3’ ends on dsDNA and mRNA. You may need to make a messy draft of this drawing as you are working through the details. Draw the TTS as it would fold after it has been transcribed and show basepairing between the mRNA and the template strand that is important for TC termination. Explain how this structure stops transcription.
35.Draw a bacterial gene (dsDNA and mRNA) showing the promoter (-35 and -10 consensus sense strand sequences), the TC start site (TSS), start of TL, open reading frame (ORF), end of TL, TC termination site (TTS), 5’-UTR, and 3’-UTR. Some of these landmarks are on the dsDNA, some on the mRNA. The TSS and TTS can be drawn on both. Indicate 5’ and 3’ ends on dsDNA and mRNA. You may need to make a messy draft of this drawing as you are working through the details. Draw the TTS as it would fold after it has been transcribed and show basepairing between the mRNA and the template strand that is important for TC termination. Explain how this structure stops transcription.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
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35.Draw a bacterial gene (dsDNA and mRNA) showing the promoter (-35 and -10 consensus sense strand sequences), the TC start site (TSS), start of TL, open reading frame (ORF), end of TL, TC termination site (TTS), 5’-UTR, and 3’-UTR. Some of these landmarks are on the dsDNA, some on the mRNA. The TSS and TTS can be drawn on both. Indicate 5’ and 3’ ends on dsDNA and mRNA. You may need to make a messy draft of this drawing as you are working through the details.
Draw the TTS as it would fold after it has been transcribed and show basepairing between the mRNA and the template strand that is important for TC termination. Explain how this structure stops transcription.
Expert Solution
Step 1: Introduction
The bacterial genome consists of a single chromosome and plasmids. The chromosome is double-stranded DNA with a homogeneous distribution of genes. The process by which the m RNA transcript is synthesized by the double-stranded DNA is known as transcription. The enzyme that catalyzes this process is known as RNA polymerase.
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