3. The rates of a carbonic anhydrase catalyzed reaction of CO2 and H20, in the absence and presence of an inhibitor (I), are measured and given below. The initial concentration of the enzyme carbonic anhydrase was 20 mM, and the initial concentration of the inhibitor concentration was 6.0 mM. (Attach any necessary graphs) Ro (M s) Ro (M s²) [S]. (M) No inhibitor With Inhibitor 1.00E-03 1.76E-06 1.17E-06 2.00E-03 2.50E-06 1.45E-06 5.00E-03 3.34E-06 1.70E-06 1.00E-02 3.77E-06 1.81E-06 2.00E-02 4.02E-06 1.86E-06 a) Determine the mode of inhibition b) Calculate the values of Km, K, Rmax, k2.

Biochemistry
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Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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3. The rates of a carbonic anhydrase catalyzed reaction of CO2 and H20, in the absence and
presence of an inhibitor (I), are measured and given below. The initial concentration of the
enzyme carbonic anhydrase was 20 mM, and the initial concentration of the inhibitor
concentration was 6.0 mM. (Attach any necessary graphs)
R. (M s)
Ro (M s²)
[S]. (M)
No inhibitor
With Inhibitor
1.00E-03
1.76E-06
1.17E-06
2.00E-03
2.50E-06
1.45E-06
5.00E-03
3.34E-06
1.70E-06
1.00E-02
3.77E-06
1.81E-06
2.00E-02
4.02E-06
1.86E-06
a) Determine the mode of inhibition
b) Calculate the values of Km, Ki, Rmax, k2.
Transcribed Image Text:3. The rates of a carbonic anhydrase catalyzed reaction of CO2 and H20, in the absence and presence of an inhibitor (I), are measured and given below. The initial concentration of the enzyme carbonic anhydrase was 20 mM, and the initial concentration of the inhibitor concentration was 6.0 mM. (Attach any necessary graphs) R. (M s) Ro (M s²) [S]. (M) No inhibitor With Inhibitor 1.00E-03 1.76E-06 1.17E-06 2.00E-03 2.50E-06 1.45E-06 5.00E-03 3.34E-06 1.70E-06 1.00E-02 3.77E-06 1.81E-06 2.00E-02 4.02E-06 1.86E-06 a) Determine the mode of inhibition b) Calculate the values of Km, Ki, Rmax, k2.
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