2. An enzyme-catalyzed reaction is carried out at several different substrate concentrations (listed below), either (1) with no inhibitor, or (2) in the presence of an inhibitor, I, at 0.63 g/L. Given the following data, determine: A. what type of inhibitor I is B. the value of K₁ No Inhibitor: With Inhibitor: Substrate Concentration (g/L) 0.86 2.35 3.80 4.90 6.10 18.4 Reaction Velocity (g/L-s) 0.179 0.398 0.567 0.666 0.726 0.950 0.322 0.407 0.443 0.495 0.639 Reaction Velocity (g/L-s) 0.161

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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2.
An enzyme-catalyzed reaction is carried out at several different substrate concentrations (listed below), either
(1) with no inhibitor, or (2) in the presence of an inhibitor, I, at 0.63 g/L. Given the following data, determine:
A. what type of inhibitor I is
B. the value of Ki
No Inhibitor:
With Inhibitor:
Substrate Concentration (g/L) 0.86
Reaction Velocity (g/L-s)
Reaction Velocity (g/L-s)
0.179
0.161
2.35 3.80 4.90 6.10 18.4
0.398 0.567 0.666 0.726 0.950
0.322 0.407 0.443 0.495 0.639
Transcribed Image Text:2. An enzyme-catalyzed reaction is carried out at several different substrate concentrations (listed below), either (1) with no inhibitor, or (2) in the presence of an inhibitor, I, at 0.63 g/L. Given the following data, determine: A. what type of inhibitor I is B. the value of Ki No Inhibitor: With Inhibitor: Substrate Concentration (g/L) 0.86 Reaction Velocity (g/L-s) Reaction Velocity (g/L-s) 0.179 0.161 2.35 3.80 4.90 6.10 18.4 0.398 0.567 0.666 0.726 0.950 0.322 0.407 0.443 0.495 0.639
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