6. Malate dehydrogenase catalyzes the following reversible reaction: NAD+ NADH + H* IZI malate dehydrogenase COO- HO-C-H CH₂ COO™ L-Malate COO- CH₂ Malate + NAD →NADH + H+ + oxaloacetate COO- Oxaloacetate AG' = 29.7 kJ/mol Calculate AG" and the ratio or products and reactants for the malate dehydrogenase reaction to proceed from left to right as shown. (The Faraday constant, , is 96.48 kJ/V-mol; RT(37°C) = 2.58kJ/mol) Steps: 1. Explain how you determined which molecule is an electron donor Malate and which is an acceptor NAD*. -2- 2. Calculate AED (write equation, then show calculations, for standard reduction potentials (E_values) see table in the posted lecture) 3. Calculate AG (write equation, then show calculations) 4. Calculate the ratio of products and reactants needed to for Malate + NAD+→NADH + H+ + oxaloacetate reaction to proceed forward (write equation, then show calculations)
6. Malate dehydrogenase catalyzes the following reversible reaction: NAD+ NADH + H* IZI malate dehydrogenase COO- HO-C-H CH₂ COO™ L-Malate COO- CH₂ Malate + NAD →NADH + H+ + oxaloacetate COO- Oxaloacetate AG' = 29.7 kJ/mol Calculate AG" and the ratio or products and reactants for the malate dehydrogenase reaction to proceed from left to right as shown. (The Faraday constant, , is 96.48 kJ/V-mol; RT(37°C) = 2.58kJ/mol) Steps: 1. Explain how you determined which molecule is an electron donor Malate and which is an acceptor NAD*. -2- 2. Calculate AED (write equation, then show calculations, for standard reduction potentials (E_values) see table in the posted lecture) 3. Calculate AG (write equation, then show calculations) 4. Calculate the ratio of products and reactants needed to for Malate + NAD+→NADH + H+ + oxaloacetate reaction to proceed forward (write equation, then show calculations)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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