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1. Lyticase is an enzyme that breaks down cell membranes. It is particularly useful when harvesting proteins made in yeast or bacteria cells. In a series of 6 experiments, lyticase was used to break down cells at 40 °C, using different substrate concentrations. Determine the parameters from the Michaelis-Menten equation, Km and Vmax, for the reaction with data given below: (A) use a Lineweaver-Burk plot (B) use an Eadie-Hofstee plot (C) use a Hanes-Woolf plot (see textbook) [S], mmol/L 8.400 2.310 1.290 0.630 0.201 0.0720 reaction? v, mmol/(L-min) 0.0343 0.0254 0.0192 0.0140 0.00620 0.00250 (D) Which of the substrate concentrations used in the experiments above would achieve closest to a zero-order

1. Lyticase is an enzyme that breaks down cell membranes. It is particularly useful when harvesting proteins made in yeast or bacteria cells. In a series of 6 experiments, lyticase was used to break down cells at 40 °C, using different substrate concentrations. Determine the parameters from the Michaelis-Menten equation, Km and Vmax, for the reaction with data given below: (A) use a Lineweaver-Burk plot (B) use an Eadie-Hofstee plot (C) use a Hanes-Woolf plot (see textbook) [S], mmol/L 8.400 2.310 1.290 0.630 0.201 0.0720 reaction? v, mmol/(L-min) 0.0343 0.0254 0.0192 0.0140 0.00620 0.00250 (D) Which of the substrate concentrations used in the experiments above would achieve closest to a zero-order

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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1. Lyticase is an enzyme that breaks down cell membranes. It is particularly useful when harvesting proteins made in yeast or bacteria cells. In a series of 6 experiments, lyticase was used to break down cells at 40 °C, using different substrate concentrations. Determine the parameters from the Michaelis-Menten equation, Km and Vmax, for the reaction with data given below: (A) use a Lineweaver-Burk plot (B) use an Eadie-Hofstee plot (C) use a Hanes-Woolf plot (see textbook) [S], mmol/L 8.400 2.310 1.290 0.630 0.201 0.0720 reaction? v, mmol/(L-min) 0.0343 0.0254 0.0192 0.0140 0.00620 0.00250 (D) Which of the substrate concentrations used in the experiments above would achieve closest to a zero-order Note: when doing a trendline curve fit for data, if the equation coefficients show up with less than 3 significant figures (the slope and intercept), you should adjust the trendline equation format to switch to scientific or number with enough places after the decimal to get an accurate answer with at least the same number of significant figures as in the experimental data.
Transcribed Image Text:1. Lyticase is an enzyme that breaks down cell membranes. It is particularly useful when harvesting proteins made in yeast or bacteria cells. In a series of 6 experiments, lyticase was used to break down cells at 40 °C, using different substrate concentrations. Determine the parameters from the Michaelis-Menten equation, Km and Vmax, for the reaction with data given below: (A) use a Lineweaver-Burk plot (B) use an Eadie-Hofstee plot (C) use a Hanes-Woolf plot (see textbook) [S], mmol/L 8.400 2.310 1.290 0.630 0.201 0.0720 reaction? v, mmol/(L-min) 0.0343 0.0254 0.0192 0.0140 0.00620 0.00250 (D) Which of the substrate concentrations used in the experiments above would achieve closest to a zero-order Note: when doing a trendline curve fit for data, if the equation coefficients show up with less than 3 significant figures (the slope and intercept), you should adjust the trendline equation format to switch to scientific or number with enough places after the decimal to get an accurate answer with at least the same number of significant figures as in the experimental data.
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