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) In the thin layer chromatography of practical 5 the chromatography tank contained a mixture of n-butanol:acetic acid:water (2:1:1) as the developing solvent. i) predict what would happen to the migration of the sugars if you would use butane:acetic acid:water (10:1:1) as the developing solvent; and ii) provide a rationale for your prediction that takes into account the principle of TLC separation, and the chemical properties of the sugars. The chemical spot tests for carbohydrates and reducing sugars on these samples as outlined below: 1.Glucose 2. Sucrose 3. Fructose 4. Unknown 5. Lactose 6. Galactose 7. Maltose 8. Honey 9. Ripe banana 10. Starch 11. Distilled water (control)

) In the thin layer chromatography of practical 5 the chromatography tank contained a mixture of n-butanol:acetic acid:water (2:1:1) as the developing solvent. i) predict what would happen to the migration of the sugars if you would use butane:acetic acid:water (10:1:1) as the developing solvent; and ii) provide a rationale for your prediction that takes into account the principle of TLC separation, and the chemical properties of the sugars. The chemical spot tests for carbohydrates and reducing sugars on these samples as outlined below: 1.Glucose 2. Sucrose 3. Fructose 4. Unknown 5. Lactose 6. Galactose 7. Maltose 8. Honey 9. Ripe banana 10. Starch 11. Distilled water (control)

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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) In the thin layer chromatography of practical 5 the chromatography tank contained a mixture of n-butanol:acetic acid:water (2:1:1) as the developing solvent.

  1. i) predict what would happen to the migration of the sugars if you would use butane:acetic acid:water (10:1:1) as the developing solvent; and ii) provide a rationale for your prediction that takes into account the principle of TLC separation, and the chemical properties of the sugars. The chemical spot tests for carbohydrates and reducing sugars on these
    samples as outlined below:
    1.Glucose
    2. Sucrose
    3. Fructose
    4. Unknown
    5. Lactose
    6. Galactose
    7. Maltose
    8. Honey
    9. Ripe banana
    10. Starch
    11. Distilled water (control)

     

 

0.cum 7 2 .lom 2.5cm S 3.5cm 3.2cm 3.5em Unknin Fructose 3.5 см. С gavactore ( to ose) C 3.1 cm 2.8cm 3.5cm 3 con 3.5/6.5=0.54 (m 3:2165=0.49 CK 3-5165=054 (n 3.5/6-5=0.54 2.5/6.5=0.4m 2.516.5=038 3.4/6.5=0.52 2.8/6.5=0.43 55/65=054 3.4/6.5 = 0.52 1.5.5:m Si
Transcribed Image Text:0.cum 7 2 .lom 2.5cm S 3.5cm 3.2cm 3.5em Unknin Fructose 3.5 см. С gavactore ( to ose) C 3.1 cm 2.8cm 3.5cm 3 con 3.5/6.5=0.54 (m 3:2165=0.49 CK 3-5165=054 (n 3.5/6-5=0.54 2.5/6.5=0.4m 2.516.5=038 3.4/6.5=0.52 2.8/6.5=0.43 55/65=054 3.4/6.5 = 0.52 1.5.5:m Si
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