54. The kinetics of a bacterial dehalogenase were investigated. Cal-culate Ky and Vmax from the following data:[S] (mM)Vo (nmol · min-1)0.040.2290.130.4930.400.7550.900.8801.300.917

Enzymes are biological catalysts that help in increasing the reaction rate. They do not get used up…

Answered: 54. The kinetics of a bacterial…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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You are evaluating the kinetics of an enzyme catalyzed reaction containing 5.5 μM total enzyme and 11.2 μM substrate. At this substrate concentration, you determine that the Vo = 88.6 μmol mL-¹. s-¹. If the Vmax 833.3 mM s the KM is: . == " 30.9 μΜ 10.4 μΜ Ο 124.6 μΜ 234 μΜ 94.5 μM
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54. The kinetics of a bacterial dehalogenase were investigated. culate Ky and Vmax from the following data: [S] (mM) Vo (nmol · min-1) 0.04 0.229 0.13 0.493 0.40 0.755 0.90 0.880 1.30 0.917