Calculate the values of the

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
8
e.Consider the reversible enzyme reaction
wwm m
F+H2O ==== M
Where F=Fumarate and M=Malonate
At 25 C and Ph 8 the maximum velocities V and Michaellis constants of these reactions are :
(0.6 × 10ª s-1)(E).
V = (0.2 x 10³ s-1) (E).
K, = 7 x 10-6 mol L-1
VM =
KM = 100 x 10-6 mol L-1
%3D
%3D
where (E), is the total molar concentration of enzymatic sites. Calculate the values of the
four rate constants in the mechanism
ka
E + F EX E + M
ka
ka
Show your work.
Transcribed Image Text:e.Consider the reversible enzyme reaction wwm m F+H2O ==== M Where F=Fumarate and M=Malonate At 25 C and Ph 8 the maximum velocities V and Michaellis constants of these reactions are : (0.6 × 10ª s-1)(E). V = (0.2 x 10³ s-1) (E). K, = 7 x 10-6 mol L-1 VM = KM = 100 x 10-6 mol L-1 %3D %3D where (E), is the total molar concentration of enzymatic sites. Calculate the values of the four rate constants in the mechanism ka E + F EX E + M ka ka Show your work.
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