Homework Help is Here – Start Your Trial Now!
Science
Biology
2. You are excited because you discovered a DNA sequence motif that appears to be in the promoters of very highly expressed genes. You suspect that it is the binding site for a transcription factor (TF). The problem is, that you can't identify the TF! The motif you found is: A-A-T-C-G-C/G-A/T-A/T-C/G-A/T where 'X' is any nucleotide and '/' denotes either of the two nucleotides. a) You give up trying to identify the TF and decide that you are going to engineer your own TF. How might you go about synthetically creating a novel TF that best targets this sequence? What considerations would you need to take into account here? b) How might you go about confirming that this sequence is indeed important for gene expression?

2. You are excited because you discovered a DNA sequence motif that appears to be in the promoters of very highly expressed genes. You suspect that it is the binding site for a transcription factor (TF). The problem is, that you can't identify the TF! The motif you found is: A-A-T-C-G-C/G-A/T-A/T-C/G-A/T where 'X' is any nucleotide and '/' denotes either of the two nucleotides. a) You give up trying to identify the TF and decide that you are going to engineer your own TF. How might you go about synthetically creating a novel TF that best targets this sequence? What considerations would you need to take into account here? b) How might you go about confirming that this sequence is indeed important for gene expression?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Topic Video
Question
2. You are excited because you discovered a DNA sequence motif that appears to be in the promoters of very highly expressed genes. You suspect that it is the binding site for a transcription factor (TF). The problem is, that you can't identify the TF! The motif you found is: A-A-T-C-G-C/G-A/T-A/T-C/G-A/T where 'X' is any nucleotide and '/' denotes either of the two nucleotides. a) You give up trying to identify the TF and decide that you are going to engineer your own TF. How might you go about synthetically creating a novel TF that best targets this sequence? What considerations would you need to take into account here? b) How might you go about confirming that this sequence is indeed important for gene expression?
Transcribed Image Text:2. You are excited because you discovered a DNA sequence motif that appears to be in the promoters of very highly expressed genes. You suspect that it is the binding site for a transcription factor (TF). The problem is, that you can't identify the TF! The motif you found is: A-A-T-C-G-C/G-A/T-A/T-C/G-A/T where 'X' is any nucleotide and '/' denotes either of the two nucleotides. a) You give up trying to identify the TF and decide that you are going to engineer your own TF. How might you go about synthetically creating a novel TF that best targets this sequence? What considerations would you need to take into account here? b) How might you go about confirming that this sequence is indeed important for gene expression?
Expert Solution
steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Gene expression
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS