2. In order to make a recombinant plasmid for transformation, EcoRI you digested a bacterial plasmid with restriction enzyme EcoRI. You want to insert a gene of interest that codes for 5... GAATTC...3' antibiotic resistance. You decide to try both XXRI and ZZRI 3... CTTAAG...5 restrictions enzymes for the antibiotic resistance gene. For XXRI, would you expect the gene insertion process to work? Why or why not? If you grow your transformed bacteria (assuming all other parts of a. XXRI 5... GAATTC...3 3... СТТАAG...5' transformation work), what would you expect to see on an LB/antibiotic plate? b. For ZZRI, would you expect the gene insertion process ZZRI to work? Why or why not? If you grow your transformed bacteria (assuming all other parts of 5... A A ATTT...3 3... TTTA AA... 5 transformation work), what would you expect to see on an LB/antibiotic plate?
2. In order to make a recombinant plasmid for transformation, EcoRI you digested a bacterial plasmid with restriction enzyme EcoRI. You want to insert a gene of interest that codes for 5... GAATTC...3' antibiotic resistance. You decide to try both XXRI and ZZRI 3... CTTAAG...5 restrictions enzymes for the antibiotic resistance gene. For XXRI, would you expect the gene insertion process to work? Why or why not? If you grow your transformed bacteria (assuming all other parts of a. XXRI 5... GAATTC...3 3... СТТАAG...5' transformation work), what would you expect to see on an LB/antibiotic plate? b. For ZZRI, would you expect the gene insertion process ZZRI to work? Why or why not? If you grow your transformed bacteria (assuming all other parts of 5... A A ATTT...3 3... TTTA AA... 5 transformation work), what would you expect to see on an LB/antibiotic plate?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Concept explainers
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Question
Transcribed Image Text:2. In order to make a recombinant plasmid for transformation,
ЕcoRI
you digested a bacterial plasmid with restriction enzyme
5'... GAATTC...3'
EcoRI. You want to insert a gene of interest that codes for
antibiotic resistance. You decide to try both XXRI and ZZRI
3... CTTAAG...5'
restrictions enzymes for the antibiotic resistance gene.
For XXRI, would you expect the gene insertion process
to work? Why or why not? If you grow your
transformed bacteria (assuming all other parts of
а.
XXRI
5... GAATTC...3
3..... СТТАA G...5
transformation work), what would you expect to see
on an LB/antibiotic plate?
b. For ZZRI, would you expect the gene insertion process
to work? Why or why not? If you grow your
ZZRI
transformed bacteria (assuming all other parts of
transformation work), what would you expect to see
on an LB/antibiotic plate?
5... A A ATTL...3'
3... TTTA A A... 5
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education