2. Below is experimental data regarding mode of inhibition of "Enzyme K" by sucralose. This is a double- reciprocal plot of enzyme velocity (Vo) versus substrate concentration, and each line represents a series of data collected at a different sucralose concentration. This is preliminary experimental data, so for a first- approximation of the experimental data, velocity data without inhibitor present can be overlooked (ignored). 0.04 0 0 mM sucralose 0.035 • 2.5 mM sucralose 0.03 • 10 mM sucralose 0.025 0.02 ...... - 0.015 0.01 0.005 -6 2 4 -0.005 -0.01 1/ [substrate] (mM) A) Propose a mechanism of inhibition of this enzyme by sucralose. B) Explain your reasoning to part A). 1/V. (mM-1 min)

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Chapter1: Biochemistry: An Evolving Science
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2. Below is experimental data regarding mode of inhibition of "“Enzyme K" by sucralose. This is a double-
reciprocal plot of enzyme velocity (Vo) versus substrate concentration, and each line represents a series of data
collected at a different sucralose concentration. This is preliminary experimental data, so for a first-
approximation of the experimental data, velocity data without inhibitor present can be overlooked (ignored).
0.04
00 mM sucralose
0.035
O 2.5 mM sucralose
0.03
O 10 mM sucralose
0.025
0.02
0.015
0.01
0.005
-6
2
4
-0.005
-0.01
1/ [substrate] (mM)
A) Propose a mechanism of inhibition of this enzyme by sucralose.
B) Explain your reasoning to part A).
1/V. (mM1 min)
Transcribed Image Text:2. Below is experimental data regarding mode of inhibition of "“Enzyme K" by sucralose. This is a double- reciprocal plot of enzyme velocity (Vo) versus substrate concentration, and each line represents a series of data collected at a different sucralose concentration. This is preliminary experimental data, so for a first- approximation of the experimental data, velocity data without inhibitor present can be overlooked (ignored). 0.04 00 mM sucralose 0.035 O 2.5 mM sucralose 0.03 O 10 mM sucralose 0.025 0.02 0.015 0.01 0.005 -6 2 4 -0.005 -0.01 1/ [substrate] (mM) A) Propose a mechanism of inhibition of this enzyme by sucralose. B) Explain your reasoning to part A). 1/V. (mM1 min)
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