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1. The initial rate of an enzymatic reaction was determined at different substrate concentrations. The data is below: [S] (μmoles/L) v [(μmol/L) min -1] 20 50 100 200 65 102 120 135 A. Graph Michaelis and Menten and use it to estimate Vmax and Km. B. Make the Lineweaver-Burk plot and use it to estimate Vmax and Km. C. What would you expect to find in the values of Vmax and Km if you do the same experiment but with twice the amount of enzyme used here. Draw your answer on the MM graph.

1. The initial rate of an enzymatic reaction was determined at different substrate concentrations. The data is below: [S] (μmoles/L) v [(μmol/L) min -1] 20 50 100 200 65 102 120 135 A. Graph Michaelis and Menten and use it to estimate Vmax and Km. B. Make the Lineweaver-Burk plot and use it to estimate Vmax and Km. C. What would you expect to find in the values of Vmax and Km if you do the same experiment but with twice the amount of enzyme used here. Draw your answer on the MM graph.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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1. The initial rate of an enzymatic reaction was determined at different substrate concentrations. The data is below: [S] (μmoles/L) v [(μmol/L) min -¹] 20 50 100 200 65 102 120 135 A. Graph Michaelis and Menten and use it to estimate Vmax and Km. B. Make the Lineweaver-Burk plot and use it to estimate Vmax and Km. C. What would you expect to find in the values of Vmax and Km if you do the same experiment but with twice the amount of enzyme used here. Draw your answer on the MM graph. 2. The Table below shows the data collected by an undergraduate student of the enzyme rate at different substrate concentrations. In the presence and in the absence of an inhibitor.
Transcribed Image Text:1. The initial rate of an enzymatic reaction was determined at different substrate concentrations. The data is below: [S] (μmoles/L) v [(μmol/L) min -¹] 20 50 100 200 65 102 120 135 A. Graph Michaelis and Menten and use it to estimate Vmax and Km. B. Make the Lineweaver-Burk plot and use it to estimate Vmax and Km. C. What would you expect to find in the values of Vmax and Km if you do the same experiment but with twice the amount of enzyme used here. Draw your answer on the MM graph. 2. The Table below shows the data collected by an undergraduate student of the enzyme rate at different substrate concentrations. In the presence and in the absence of an inhibitor.
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