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- 13. How would ATP production in an aerobic yeast culture change if we add the following metabolites or chemicals to the cultivation media? Explain why in each case by citing which enzyme(s) or process(es) would be affected. I Chemical added: Effect: succinyl-CoA ADP Citrate fructose-2,6- bisphosphate DCCD FCCP (none/enhance/ decrease) Reason:I am needing help with 1 through 3, please 1) Using the graphs, indicate if the data supports or does not support the hypothesis: The rate of balloon inflation will be directly proportional to the amount of sugar added to the yeast solution. Also, which graph illustrates the most clearly what happened in the experiment and why? 2) Which graph illustrates the most clearly what happened in the experiment? Why? 3) Glycolysis in an anaerobic environment produces a net of 2 ATP and Pyruvate. Why does Yeast bother to ferment Pyruvate to Ethanol?5. STOICHIOMETRY OF CELL GROWTH. Candida utilis is a yeast that is approved for use as a food additive, and is used in industrial production of carotenoids and other biochemicals. A batch cell culture of Candida utilis growing on glucose (C6H12O6) and ammonia (NH3) is used to grow additional cells under aerobic conditions. Given feeds of 550 g glucose, 230 g ammonia and 280 g oxygen (O₂) (assume O₂ is bubbled through so that all 280 g are easily accessible to the cells}: A. Which nutrient is the limiting nutrient? B. What mass of new cells (dry basis) will be produced when the limiting nutrient is fully consumed? (Assume that the cells stay in exponential growth phase until all of this nutrient is consumed.) C. Write the balanced stoichiometric equation for cell growth. D. What is the respiratory quotient (RQ) for this cell growth? Notes: There are two values for cell formula for Candida Utilis growing on glucose. Use the first entry with a specific growth rate of 0.08 h¹. Yields based…
- 1. Demonstrate your understanding whether carrier-free biocatalysts should or should not be considered as good biocatalysts. You discussion must be in detail and related to biocatalysis. You can provide detail explanation or provide example. 2. Compare and contrast the following pairs of terms. Relate their similarities and differences to applications in biocatalysis. Zymonomas sp. and Saccharomyces sp.Main products of yeast fermentation The main products of yeast fermentation are (Check all that apply) Check All That Apply alcohol FADH CO CO2 lactic acid NAD+ NADH pyruvate1. A yeast variant was discovered that had a shorter glycolytic pathway. This "mutant" yeast made a novel enzyme that catalyzed the following reaction: Glyceraldehyde-3-phosphate + H; + NAD* E→3-phosphoglycerate + NADH + H* (HINT: Examine ATP production from glucose in each case below.) A. Would you expect this novel yeast strain to grow well under anaerobic conditions using glucose as a carbon source? Explain. B. Would you expect this novel yeast strain to grow well under aerobic conditions using glucose as a carbon source? Explain.
- The main products of yeast fermentation are (Check all that apply) Check All That Apply alcohol FADH CO CO2 lactic acid NAD+ NADH pyruvatel l l l M cell structure Cin chemostat is fed with sugar The growth rate follows the process equation VscS+C2C + Vp VPC о where P is the useful product The rate law followed is as determined experimentally rg = kg [C] [S] 1. (S) Orite down the mass balances for S, C, P and I, assuming feed contains ody the substrate of concentration [5]b] Solve them for [S], [C] and [P],10_UG_26563_ch09_167-184.indd 175 Press esc to exit full screen Yeast Fermentation The unicellular fungus Saccharomyces cerevisiae is used in the food industry to ferment sugars. The rate of fermentation is measured by CO₂ production. The addition of inorganic phosphate increases this rate. THINK Answer this question individually: 1. Why does the fermentation of sugars, such as glucose and fructose, depend upon the presence of P? PAIR Answer the following questions with a partner: 2. Which glycolytic reactions require P? 3. Several glycolytic intermediates contain phosphate. Where did these groups come from during the reactions? SHARE 4. How much P, is required to generate one CO₂? As a class, discuss the following questions: 5. Is CO₂ generation the most reliable way to measure ethanol fermentation? Why or why not? HANDOUT 175 23/03/17 2:02 pm Page 1 of 4 ...... ZOOM + K
- 5. How does yeast utilize glucose for respiration? Write down the chemical equation msi1. How would ATP production in an aerobic yeast culture change if we add the following metabolites or chemicals to the cultivation media? Explain why in each case by citing which enzyme(s) or process(es) would be affected.What is the end product of anaerobic respiration of yeast? O Pyruvate O Lactate O Alcohol O Glucose