5. STOICHIOMETRY OF CELL GROWTH. Candida utilis is a yeast that is approved for use as a food additive, and is used in industrial production of carotenoids and other biochemicals. A batch cell culture of Candida utilis growing on glucose (C6H12O6) and ammonia (NH3) is used to grow additional cells under aerobic conditions. Given feeds of 550 g glucose, 230 g ammonia and 280 g oxygen (O₂) (assume O₂ is bubbled through so that all 280 g are easily accessible to the cells): A. Which nutrient is the limiting nutrient? B. What mass of new cells (dry basis) will be produced when the limiting nutrient is fully consumed? (Assume that the cells stay in exponential growth phase until all of this nutrient is consumed.) C. Write the balanced stoichiometric equation for cell growth. D. What is the respiratory quotient (RQ) for this cell growth? Notes: There are two values for cell formula for Candida Utilis growing on glucose. Use the first entry with a specific growth rate of 0.08 h¹. Yields based on glucose and O₂ are in Table 6.1. The cell yield per gram of ammonia consumed (if it is the limiting reactant), YX/NH3 = 1.44 g/g.
5. STOICHIOMETRY OF CELL GROWTH. Candida utilis is a yeast that is approved for use as a food additive, and is used in industrial production of carotenoids and other biochemicals. A batch cell culture of Candida utilis growing on glucose (C6H12O6) and ammonia (NH3) is used to grow additional cells under aerobic conditions. Given feeds of 550 g glucose, 230 g ammonia and 280 g oxygen (O₂) (assume O₂ is bubbled through so that all 280 g are easily accessible to the cells): A. Which nutrient is the limiting nutrient? B. What mass of new cells (dry basis) will be produced when the limiting nutrient is fully consumed? (Assume that the cells stay in exponential growth phase until all of this nutrient is consumed.) C. Write the balanced stoichiometric equation for cell growth. D. What is the respiratory quotient (RQ) for this cell growth? Notes: There are two values for cell formula for Candida Utilis growing on glucose. Use the first entry with a specific growth rate of 0.08 h¹. Yields based on glucose and O₂ are in Table 6.1. The cell yield per gram of ammonia consumed (if it is the limiting reactant), YX/NH3 = 1.44 g/g.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:6.
Repeat problem 5 for Candida utilis growing on ethanol as the carbon source (all other nutrient sources
the same as # 5). Feeds are 550 g ethanol, 230 g ammonia and 280 g oxygen (O₂), and YX/NH3, = 1.44 g/g. Use the
cell composition corresponding to a specific growth rate on ethanol of 0.06 h-¹.
Transcribed Image Text:5.
STOICHIOMETRY OF CELL GROWTH. Candida utilis is a yeast that is approved for use as a food additive, and
is used in industrial production of carotenoids and other biochemicals. A batch cell culture of Candida utilis
growing on glucose (C6H12O6) and ammonia (NH3) is used to grow additional cells under aerobic conditions.
Given feeds of 550 g glucose, 230 g ammonia and 280 g oxygen (O₂) (assume O₂ is bubbled through so that all
280 g are easily accessible to the cells}:
A. Which nutrient is the limiting nutrient?
B. What mass of new cells (dry basis) will be produced when the limiting nutrient is fully consumed?
(Assume that the cells stay in exponential growth phase until all of this nutrient is consumed.)
C. Write the balanced stoichiometric equation for cell growth.
D. What is the respiratory quotient (RQ) for this cell growth?
Notes: There are two values for cell formula for Candida Utilis growing on glucose. Use the first entry with a specific
growth rate of 0.08 h¹. Yields based on glucose and O₂ are in Table 6.1. The cell yield per gram of ammonia consumed (if
it is the limiting reactant), YX/NH3 = 1.44 g/g.
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