5.STOICHIOMETRY OF CELL GROWTH. Candida utilis is a yeast that is approved for use as a food additive, andis used in industrial production of carotenoids and other biochemicals. A batch cell culture of Candida utilisgrowing on glucose (C6H12O6) and ammonia (NH3) is used to grow additional cells under aerobic conditions.Given feeds of 550 g glucose, 230 g ammonia and 280 g oxygen (O₂) (assume O₂ is bubbled through so that all280 g are easily accessible to the cells}:A. Which nutrient is the limiting nutrient?B. What mass of new cells (dry basis) will be produced when the limiting nutrient is fully consumed?(Assume that the cells stay in exponential growth phase until all of this nutrient is consumed.)C. Write the balanced stoichiometric equation for cell growth.D. What is the respiratory quotient (RQ) for this cell growth?Notes: There are two values for cell formula for Candida Utilis growing on glucose. Use the first entry with a specificgrowth rate of 0.08 h¹. Yields based on glucose and O₂ are in Table 6.1. The cell yield per gram of ammonia consumed (ifit is the limiting reactant), YX/NH3 = 1.44 g/g.

INTRODUCTION In order for cells to grow, they require certain nutrients such as glucose, ammonia,…

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Human Anatomy & Physiology (11th Edition)

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There are two values for cell formula for Candida Utilis growing on glucose. Use the first entry with a specific rate of 0.08 h¹. Yields based on glucose and O₂ are in Table 6.1. The cell yield per gram of ammonia consumed (if limiting reactant), YX/NH3 = 1.44 g/g.