Hurray! The bacteria did their job and expressed your protein. Now, you need to isolate biochemisfunase from the other bacterial proteins. To do this, you first need to break open the bacterial cells, a process called cell lysis. The lysis buffer you are going to use requires 225 mM potassium chloride (KCl), which is available as a crystalline solid. The lysis buffer also requires 22.5% (v/v) glycerol, which is available as a 100% stock solution. When making your lysis buffer,you added too much water,and you ended up with 375 ml of lysis buffer solution. The postdoc you are working with, underpaid and overworked, tells you to “rescue” the solution rather than remaking it. This same postdoc tells you that concentration is more important than the final volume. How would you go about rescuing the solution? (To make the required lysis buffer, take 56.25 mL of the 100% glycerol stock and add 193.75 mL water to it.)
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Hurray! The bacteria did their job and expressed your protein. Now, you need to isolate biochemisfunase from the other bacterial proteins. To do this, you first need to break open the bacterial cells, a process called cell lysis. The lysis buffer you are going to use requires 225 mM potassium chloride (KCl), which is available as a crystalline solid. The lysis buffer also requires 22.5% (v/v) glycerol, which is available as a 100% stock solution.
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When making your lysis buffer,you added too much water,and you ended up with 375 ml of lysis buffer solution. The postdoc you are working with, underpaid and overworked, tells you to “rescue” the solution rather than remaking it. This same postdoc tells you that concentration is more important than the final volume. How would you go about rescuing the solution? (To make the required lysis buffer, take 56.25 mL of the 100% glycerol stock and add 193.75 mL water to it.)
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