You have learned quite a number of different physicochemical methods for fractionating the contents of cellular extracts and the proteins contained within them. • Match each stated goal to the most relevant method, technique, or procedure for achieving that goal. a) Removing K+ ions from a protein sample: [Select] b) Removing large cellular debris from the initial cell lysate: [Select] c) Isolating small proteins from a solution that also contains large proteins: [Select] d) Separating two proteins that differ greatly in their ionic solubility: [Select] e) Separating two proteins that differ by the magnitude of charge on their surface: [Select]

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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isoelectric focusing
gel filtration
salting out
differential centrifugation
Edman degradation
ion exchange
dialysis

You have learned quite a number of different physicochemical methods for fractionating the
contents of cellular extracts and the proteins contained within them.
• Match each stated goal to the most relevant method, technique, or procedure for achieving that
goal.
a) Removing K+ ions from a protein sample: [Select]
b) Removing large cellular debris from the initial cell lysate: [Select]
c) Isolating small proteins from a solution that also contains large proteins:
[Select]
d) Separating two proteins that differ greatly in their ionic solubility:
[Select]
e) Separating two proteins that differ by the magnitude of charge on their surface:
[Select]
Transcribed Image Text:You have learned quite a number of different physicochemical methods for fractionating the contents of cellular extracts and the proteins contained within them. • Match each stated goal to the most relevant method, technique, or procedure for achieving that goal. a) Removing K+ ions from a protein sample: [Select] b) Removing large cellular debris from the initial cell lysate: [Select] c) Isolating small proteins from a solution that also contains large proteins: [Select] d) Separating two proteins that differ greatly in their ionic solubility: [Select] e) Separating two proteins that differ by the magnitude of charge on their surface: [Select]
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