1.You are given a pure protein sample to characterize and provided the following information: Its molar extinction coefficient, ε280, is 0.25 liters micromole-1 cm-1 in both the folded and unfolded form Its AGO for unfolding is 1.2 kcal/mol at 37° (where RT = 0.59 kcal/mole) A) Using a 0.5 cm pathlength cell, you measure the absorbance at 280 nm of a 10-fold dilution of your pure protein in solution (by this, we mean that 100 ul of the protein sample was diluted to a final volume of 1 ml) and find A280 = 0.40. What is the original concentration of the protein before dilution? B) What is the concentration of the unfolded form of the protein in your sample?
1.You are given a pure protein sample to characterize and provided the following information: Its molar extinction coefficient, ε280, is 0.25 liters micromole-1 cm-1 in both the folded and unfolded form Its AGO for unfolding is 1.2 kcal/mol at 37° (where RT = 0.59 kcal/mole) A) Using a 0.5 cm pathlength cell, you measure the absorbance at 280 nm of a 10-fold dilution of your pure protein in solution (by this, we mean that 100 ul of the protein sample was diluted to a final volume of 1 ml) and find A280 = 0.40. What is the original concentration of the protein before dilution? B) What is the concentration of the unfolded form of the protein in your sample?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:1.You are given a pure protein sample to characterize and provided the following information:
Its molar extinction coefficient, ε280, is 0.25 liters micromole-1 cm-1 in both the folded and
unfolded form
Its AGO for unfolding is 1.2 kcal/mol at 37° (where RT = 0.59 kcal/mole)
A) Using a 0.5 cm pathlength cell, you measure the absorbance at 280 nm of a 10-fold dilution
of your pure protein in solution (by this, we mean that 100 ul of the protein sample was diluted
to a final volume of 1 ml) and find A280 = 0.40. What is the original concentration of the protein
before dilution?
B) What is the concentration of the unfolded form of the protein in your sample?
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