Why would this data be controversial MDCK cellsJurkat cells45sphingolipidwGPLcholesterouGPL4535cholesterol GPL2.5251.5150.505Fig.treaFig. 4. Lipid contents of DRMS from MDCK and Jurkat cells. Lipids from theTLC plates shown in Fig. 3 were quantified and grouped into sphingolipids(GalC + GluC+ LacC + SM + Gb, for MDCK cells, GluC+ SM for Jurkat cells,intensities taken from "C-labeled lipids), cholesterol and glycerophospholip-ids (PE + PI+ PS+ PC, intensities taken from "P-labeled lipids). Sphingolipid/glycerophospholipid and cholesterol/glycerophospholipid ratios relative tothe ratios for TCMS are shown. GPL, glycerophospholipids.withcontsolusessstreof peforber of carbon atoms and/or the number of double bonds in thefatty acid moicties, were detected. For cach species, its percentfraction of the total PC was calculated. In addition, the relativeconcentration of cach spccies was determined to allow compar-ison of the amounts present in different DRMS (see Table 1,which is published as supporting information on the PNAS website, www.pnas.org). PC spccies were then grouped according tothe degree of saturation of their fatty acids (Fig. 5).In MDCK cells,-60% of the total cell membrane PC wasmonounsaturated, <10% was fully saturated, and -30% pos-unsadeteIntof sawithnounfrompartitJurkaporticdomaiof glyresultsferentTakeMDCK cellsTCM70Tween 20as quaent detby the60Bry 50Lubral WX50Brj 98preparaarbitrary unitsTCMTweenBrij 58LubrolBrij 98Brij 96TritonCHAPSTCMTweenBrij 58LubrolBrij 98otal PCBrij 96TritonCHAPS

DRM or detergent resistant membrane is mostly prepared by applying any detergent (such as: Triton X)…

MDCK cells Jurkat cells 45 sphingolipida/GPL 45 sphngolpds/GPL cholesterolGPL 4. 4. 3.5 cholesteroGPL 35 2.5 25 1.5 15 0.5 05 Fig. 4. Lipid contents of DRMS from MDCK and Jurkat cells. Lipids from the TLC plates shown in Fig. 3 were quantified and grouped into sphingolipids (GalC + GluC+ LacC+ SM + Gb, for MDCK cells, GluC+ SM for Jurkat cells, intensities taken from "C-labeled lipids), cholesterol and glycerophospholip- ids (PE+ PI+ PS + PC, intensities taken from "P-labeled lipids). Sphingolipid/ glycerophospholipid and cholesterol/glycerophospholipid ratios relative to the ratios for TCMS are shown. GPL, glycerophospholipids. arbitrary units TCM Tween Brij 58 Lubrol Brij 98 Brij 96 Triton CHAPS TCM Tween Brij 58 Lubrol Brij 98 Brij 96 Triton CHAPS

MDCK cells Jurkat cells 45 sphingolipida/GPL 45 sphngolpds/GPL cholesterolGPL 4. 4. 3.5 cholesteroGPL 35 2.5 25 1.5 15 0.5 05 Fig. 4. Lipid contents of DRMS from MDCK and Jurkat cells. Lipids from the TLC plates shown in Fig. 3 were quantified and grouped into sphingolipids (GalC + GluC+ LacC+ SM + Gb, for MDCK cells, GluC+ SM for Jurkat cells, intensities taken from "C-labeled lipids), cholesterol and glycerophospholip- ids (PE+ PI+ PS + PC, intensities taken from "P-labeled lipids). Sphingolipid/ glycerophospholipid and cholesterol/glycerophospholipid ratios relative to the ratios for TCMS are shown. GPL, glycerophospholipids. arbitrary units TCM Tween Brij 58 Lubrol Brij 98 Brij 96 Triton CHAPS TCM Tween Brij 58 Lubrol Brij 98 Brij 96 Triton CHAPS

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter26: Synthesis And Degradation Of Nucleotides

Section: Chapter Questions

Problem 22P

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