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answer the following questions
1.What is the wavelength used for the spectrophotometer? What is the principle of the spectrophotometer?
2.What is the reagent used to detect glucose in the oh and temperature experiments? What is its principle?
3.What is the optimum temperature for invertase reactions?
4.What is the optimum ph for the invertase reactions?
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- Concentrations in biochemical systems are often very dilute. Consequently, scientific notation and logarithms are often used to express concentrations. In scientific notation, numbers are expressed as coefficient x 10" To convert a number to scientific notation, proceed as follows: 1. Move the decimal place so that there is one digit in front of the decimal. 2. Account for the moved decimal in the value of x. If the decimal moved to the right, x is negative; if it moved to the left, x is positive. A logarithm is basically an exponent. Unless otherwise indicated, a logarithm is the a of 10". The numbers after the decimal point are significant; the number before the decimal just identify the location of the decimal point for the number. Notice that it is easy to estimate a logarithm from scientific notation; it's the exponent! Logarithms are commonly used to express the concentration of H. The pH is defined as pH= log (In), where the base number is 2.303. The same general rules as logs…1. A.) Which compound acts as dehydrating agent in Molisch's test? B.) With the correct answer above, what is its chemical function? 2. A.) Which compound is present in Fehling's A? B.) With the correct answer above, what is its chemical function? 3. A.) Which compound is present in Fehling's B? B.) With the correct answer above, what is its chemical function? 4. A.) Which compound acts as oxidizing agent in Benedict's Test? B.) With the correct answer above, what is its chemical function? 5. A.) Which compound acts as dehydrating agent in Bial's Test? B.) With the correct answer above, what is its chemical function?Most of the following conclusions regarding the photo shown above are correct Except: All of the following are correct regarding the disk diffusion test photos EXCEPT : Zone of inhibition Chlorine Chlorine Chlorine O-phenylphenol Hexachlorophene O-phenylphenol Hexachlorophene Hexachlorophene O-phenylpheno Quat Quat Quat Staphylococcus aureus (gram-positive) Escherichia coli Pseudomonas aeruginosa (gram-negative) (gram-negative) Select one: O to. Gram negative bacteria are the most sensitive to chemical agents O b. Only one of the four chemicals affected Pseudomonas O c. Gram positive bacteria are the most sensitive to disinfectants O d. Hexachlorophene was effective only against Gram positive bacteria. O and. Chlorine was effective against all bacteria bacteria tested
- In sample preparation for electron microscopy, arrange the following steps in correct order A. Apply non-coagulant fixatives to kill cells instantly with minimal structural alterations. B. Wash the specimen with deionized water and then dry it by passing through increasing concentrations of ethanol. C. Expose specimens to uranyl salts to enhance the electron scattering capacity of cellular components. D. Using an ultramicrotome, make ultrathin cuts in the embedded specimen that would be penetrable to electrons. E. Allow the embedding medium to seep into the interstices of the tissue.Using a P-1000 micropipette, you transfer 800 microliter of ddH2O to a beaker and measure its weight. It turns out to be only 780 milligram; what is the percent error of this micropipette?Will UPVOTE Answer the following: 1. What is the effect of boiling in the test tube with starch solution and saliva? 2. What is anaerobic oxidation? In your observation, which is the Hydrogen acceptor in the experiment, methylene blue or milk? 3. If the sample turned to blue or black upon the addition of I2 solution, is the honey genuine or not? Explain your answer 4. What changes do you think take place as the color changes upon the addition of phenol, pyrogallol or catechol and guac solution to the potato extract? 5. Differentiate an enzyme activator from an indicator
- The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield a countable plate? How would you make this? Show your calculations. Confused as to what's implied by "which dilution"? does it mean, how many times?Sucrose + H2O(negative control): Colorless to brown solution: Blue solution Conclusion for Iodine test A. amylose present B. amylose not present Conclusion for Benedict's test A. reducing sugars not present B. reducing sugars present Sucrose + HCl(potential hydrolysis): unchanged compared to sucrose+water: Conclusion for Iodine test A. more amylose present B. LESS amylose present C. NO change Conclusion for Benedict's test a. more reducing sugar present b. less reducing sugar present c. no change Did hydrolysis of sucrose occur in the presence of acid? A. Yes B. noIn an electrochemical cell, Q = 0.00025 and K = 0.70. What can you conclude about Ecell and E°cell? Ecell is positive and E°cell is positive Ecell is negative and E°cell is positive Ecell is negative and E°cell is negative Ecell is positive and E°cell is negative
- When the yeast cells have completely re-hydrated, measure out 1.8 mL of well-mixed yeast suspension (0.2% yeast) into each of two new 15 mL centrifuge tubes. Add 200 μL of a 10% (w/v) Sodium Azide*** in YGM solution to one of the 15 mL tubes with yeast suspension. Add 200 μl of YGM to the other 15 mL tube of yeast suspension for your Control. Let both tubes incubate at room temperature for 30 min, vortexing every ~5 minutes What is the % concentration of azide in this sample during metabolic inhibition?What is the % concentration of yeast in this sample during metabolic inhibition?For the nitrate reduction test, complete the table below Reaction Gas evolution (Yes/No) Color after adding reagents Color after adding zinc dust NO3 to NO2 NO3 to N2 NO3 to ammonia No reactionThis is my data collected from a Succinate Dehyrogenase Enzyme Assay - I am asked to plot a Concentration vs Time while plotting the Resuspended and Supernatant Reaction; how can this be completed? Test Tube Concentration (uM) Absorbance @ 600nm #1 0 0.00 #2 5 0.090 #3 10 0.187 #4 20 0.368 #5 40 0.715 #6 60 1.075 Table 3. Succinate Dehydrogenase Assay @ 600 nm Time point (min): Resuspended Pellet Reaction Absorbance Supernatant Reaction Absorbance Enzyme Reaction Control Absorbance 0 43.2 44.2 46.1 3 43.3 44.8 6 43.0 44.9 9 42.6 45.2 12 43.5 45.6 15 44.0 45.7 18 43.8 45.9 21 44.0 46.4 24 44.1 46.5 27 43.9 46.7 30 43.7 46.9 43.6
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