What nutrients do the following media components contain? Peptone Yeast extract Beef extract Potato infusion Agar Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? 1.Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?Please include source What nutrients do the following media components contain?• Peptone• Yeast extract• Beef extractPotato infusion• AgarWhy should agar media be completely dissolved before they are dispensed in tubes andplates?What are the bases for pegging the temperature at 121°C for 15-30 minutes during moist heatsterilization and 180°C for two (2) hours using dry heat sterilization? 1. Can you sterilizeculture media using dry heat sterilization? Why is that so?You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug inmedia prep, sterilization and culture of microorganisms?Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is ittechnically acceptable in microbiology?

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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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What nutrients do the following media components contain?

Peptone

Yeast extract

Beef extract

Potato infusion

Agar

Why should agar media be completely dissolved before they are dispensed in tubes and plates?

What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? 1.Can you sterilize culture media using dry heat sterilization? Why is that so?

You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms?

Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?

Please include source