Sweet sorghum juice is used as sole carbon source for ethanol production by Saccharomyces cerevisiae. The overall in situ sterilisation duration is 40 minutes at 120 0C for a pilot reactor (50 L) which is filled with 60% of working volume. The non-sterile medium consists of 3.28 x 106 contaminants L-1. During the autoclaving process, you have noticed that the time taken for heating and cooling are 18 minutes and 15 minutes, respectively. As a process engineer, explain if you agree with this sterilisation practice by using quantitative analysis.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Sweet sorghum juice is used as sole carbon source for ethanol production by Saccharomyces cerevisiae. The overall in situ sterilisation duration is 40 minutes at 120 0C for a pilot reactor (50 L) which is filled with 60% of working volume. The non-sterile medium consists of 3.28 x 106 contaminants L-1. During the autoclaving process, you have noticed that the time taken for heating and cooling are 18 minutes and 15 minutes, respectively. As a process engineer, explain if you agree with this sterilisation practice by using quantitative analysis.

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