Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
Refer to the chromatograph (Figure 1) below and answer the questions that follow: What is the volumetric flow rate of the column?  What type of compound is likely to be found in peak B? Explain.  What possible buffer changes could the researcher have applied at the 30ml mark? Explain.  How would one avoid cross-contamination when using this type of purification

Refer to the chromatograph (Figure 1) below and answer the questions that follow: What is the volumetric flow rate of the column?  What type of compound is likely to be found in peak B? Explain.  What possible buffer changes could the researcher have applied at the 30ml mark? Explain.  How would one avoid cross-contamination when using this type of purification

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question

Refer to the chromatograph (Figure 1) below and answer the questions that follow:

  1. What is the volumetric flow rate of the column? 
  2. What type of compound is likely to be found in peak B? Explain. 
  3. What possible buffer changes could the researcher have applied at the 30ml mark? Explain. 
  4. How would one avoid cross-contamination when using this type of purification 
A280 - па 10 20 30 Volume (ml) B 40 50 Figure 1: Affinity chromatography of semi-purified extract on a lectin-sepharose column. Column dimensions are 2cm i.d. x 5cm, flow rate is 10cm/hr.|
Transcribed Image Text:A280 - па 10 20 30 Volume (ml) B 40 50 Figure 1: Affinity chromatography of semi-purified extract on a lectin-sepharose column. Column dimensions are 2cm i.d. x 5cm, flow rate is 10cm/hr.|
Expert Solution
steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS